PUBLICATION
Molecular Identification, Polymorphism, and Expression Analysis of Major Histocompatibility Complex Class IIA and B Genes of Turbot (Scophthalmus maximus)
- Authors
- Zhang, Y.X., and Chen, S.L.
- ID
- ZDB-PUB-060719-17
- Date
- 2006
- Source
- Marine biotechnology (New York, N.Y.) 8(6): 611-623 (Journal)
- Registered Authors
- Zhang, Yuxi
- Keywords
- cDNA, expression, major histocompatibility complex class II, MHC class IIA and B, polymorphism, turbot Scophthalmus maximus
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Fish Diseases/genetics
- Fish Diseases/microbiology
- Fish Proteins/chemistry
- Fish Proteins/genetics
- Fish Proteins/metabolism
- Flatfishes/genetics*
- Gene Expression Profiling
- Gene Expression Regulation*
- Genes, MHC Class II/genetics*
- Molecular Sequence Data
- Phylogeny
- Polymorphism, Genetic*
- Vibrio Infections/metabolism
- Vibrio Infections/microbiology
- Vibrio Infections/veterinary
- PubMed
- 16832747 Full text @ Mar. Biotechnol.
Citation
Zhang, Y.X., and Chen, S.L. (2006) Molecular Identification, Polymorphism, and Expression Analysis of Major Histocompatibility Complex Class IIA and B Genes of Turbot (Scophthalmus maximus). Marine biotechnology (New York, N.Y.). 8(6):611-623.
Abstract
Major histocompatibility complex (MHC) class II has a central role in the adaptive immune system by presenting foreign peptides to the T-cell receptor. The full lengths of MHC class II A and B cDNA were cloned from turbot by homology cloning and rapid amplification of cDNA ends polymerase chain reaction (RACE PCR), and genomic organization, molecular polymorphism, and expression of turbot class IIB gene were examined to study the function of class IIB gene in fish. The deduced amino acid sequence of turbot class II A (GenBank accession no.DQ001730) and turbot class IIB (GenBank accession no. DQ094170) had 69.8%, 67.6%, 65.5%, 59.2%, 54.5%, 52.8%, 46.2%, 46.6%, 28.3%, 28.5%, 22.2% identity and 71.5%, 70.7%, 67.1%, 68.4%, 46.7%, 53.5%, 46.7%, 50.0%, 25.2%, 29.2%, 27.6% identity with those of Japanese flounder, striped sea bass, red sea bream, cichlid, rainbow trout, Atlantic salmon, carp, zebrafish, nurse shark, mouse and human, respectively. Eleven class IIB alleles were identified from three turbot individuals. The amino acid sequence of turbot class IIB designated as Scma-DAB*0101 had 86.9%, 88.6%, 88.6%, 89.4%, 87.8%, 86.9%, 84.1%, 86.5%, 87.3%, 77.1%, and 86.9% identity with those of turbot class IIB 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12 (Scma-DAB*0201- Scma-DAB*1201), respectively. Six different class IIB alleles observed in a single individual may infer the existence of three loci at least. Semiquantitative reverse transcriptase PCR (RT-PCR) demonstrated that turbot class IIA and B were ubiquitously expressed in normal tissues. Challenge of turbot with pathogenic bacteria, Vibrio anguillarum, resulted in a significant decrease in the expression of MHC class IIB mRNA from 24 h to 48 h after infection in liver and head kidney, and a significant decrease from 24 h to 72 h after infection in spleen, followed by an increase after 96 h, respectively.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping