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ZFIN ID: ZDB-PUB-060710-8
Molecular Cloning of a Novel Gene ZAhi-1 and its Expression Analysis during Zebrafish Gametogenesis
Zhou, W., and Song, P.
Date: 2006
Source: Molecular biology reports   33(2): 111-116 (Journal)
Registered Authors: Song, Ping, Zhou, Wei
Keywords: Ahi-1, cDNA cloning, in situ hybridization, RT-PCR, zebrafish
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Carrier Proteins/genetics*
  • Carrier Proteins/metabolism*
  • Cloning, Molecular
  • Expressed Sequence Tags
  • Gametogenesis*
  • Gene Expression Regulation, Developmental
  • In Situ Hybridization
  • Mice
  • Molecular Sequence Data
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Homology, Amino Acid
  • Tissue Distribution
  • Zebrafish/embryology*
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism*
PubMed: 16817020 Full text @ Mol. Biol. Rep.
Proto-oncogen Ahi-1 is closely related to a lot of human and mouse diseases. Ahi-1 mutation will lead to leukemia in mice and Joubert syndrome in human beings. We have cloned the full cDNA sequence of Ahi-1 homologous in zebrafish, and RT-PCR results of ZAhi-1 in different tissues reveal that ZAhi-1 expressed highest in the mature gonad. In situ hybridization results of zebrafish gonad show that ZAhi-1 only expressed in the early stages' gamete cells. RT-PCR analyses of mouse Ahi-1 in different stages of spermatogenesis have been done according to the published Ahi-1 sequence, and the findings reveal that Ahi-1 is expressed in gamete of pachytene stage. It can then be safely concluded that Ahi-1 might take place in the spermatocyte from the early pachytene stage to the late pachytene stage.