PUBLICATION

Fgf16 is essential for pectoral fin bud formation in zebrafish

Authors
Nomura, R., Kamei, E., Hotta, Y., Konishi, M., Miyake, A., and Itoh, N.
ID
ZDB-PUB-060710-6
Date
2006
Source
Biochemical and Biophysical Research Communications   347(1): 340-346 (Journal)
Registered Authors
Itoh, Nobuyuki, Konishi, Morichika, Miyake, Ayumi
Keywords
Fgf, Fgf16, Zebrafish, Fin, Apical ectodermal ridge, Development, Morphogenesis
MeSH Terms
  • Animals
  • Fibroblast Growth Factors/chemistry*
  • Fibroblast Growth Factors/isolation & purification
  • Fibroblast Growth Factors/metabolism*
  • Gene Expression Regulation, Developmental/physiology
  • Limb Buds/anatomy & histology
  • Limb Buds/embryology*
  • Limb Buds/physiology*
  • Organ Specificity
  • Organogenesis/physiology*
  • Tissue Distribution
  • Zebrafish/anatomy & histology
  • Zebrafish/embryology*
  • Zebrafish/physiology*
PubMed
16815307 Full text @ Biochem. Biophys. Res. Commun.
Abstract
Zebrafish pectoral fin bud formation is an excellent model for studying morphogenesis. Fibroblast growth factors (Fgfs) and sonic hedgehog (shh) are essential for pectoral fin bud formation. We found that Fgf16 was expressed in the apical ectodermal ridge (AER) of fin buds. A knockdown of Fgf16 function resulted in no fin bud outgrowth. Fgf16 is required for cell proliferation and differentiation in the mesenchyme and the AER of the fin buds, respectively. Fgf16 functions downstream of Fgf10, a mesenchymal factor, signaling to induce the expression of Fgf4 and Fgf8 in the AER. Fgf16 in the AER and shh in the zone of polarizing activity (ZPA) interact to induce and/or maintain each other's expression. These findings have revealed that Fgf16, a newly identified AER factor, plays a crucial role in pectoral fin bud outgrowth by mediating the interactions of AER-mesenchyme and AER-ZPA.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes