PUBLICATION

GFAP transgenic zebrafish

Authors
Bernardos, R.L., and Raymond, P.A.
ID
ZDB-PUB-060616-45
Date
2006
Source
Gene expression patterns : GEP   6(8): 1007-1013 (Journal)
Registered Authors
Bernardos, Rebecca, Raymond, Pamela
Keywords
Glial fibrillary acidic protein, Müller glia, Radial glia, CNS, PNS, Zebrafish, Transgenic, GFP, Enteric nervous system, Retina, Hindbrain, Development
MeSH Terms
  • Animals
  • Animals, Genetically Modified*/embryology
  • Animals, Genetically Modified*/metabolism
  • Central Nervous System/embryology
  • Central Nervous System/metabolism
  • Embryo, Nonmammalian
  • Gene Expression
  • Glial Fibrillary Acidic Protein/genetics*
  • Green Fluorescent Proteins/metabolism
  • Neuroglia/metabolism
  • Tissue Distribution
  • Transgenes
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics
PubMed
16765104 Full text @ Gene Expr. Patterns
Abstract
We have generated transgenic zebrafish that express green fluorescent protein (GFP) in glial cells driven by the zebrafish glial fibrillary acidic protein (GFAP) regulatory elements. Transgenic lines Tg(gfap:GFP) were generated from three founders; the results presented here are from the mi2001 line. GFP expression was first visible in the living embryo at the tail bud-stage, then in the developing brain by the 5-somite-stage ( approximately 12h post-fertilization, hpf) and then spreading posteriorly along the developing spinal cord by the 12-somite stage ( approximately 15hpf). At 24hpf GFP-expressing cells were in the retina and lens. By 72hpf GFP expression levels were strong and localized to the glia of the brain, neural retina, spinal cord, and ventral spinal nerves, with moderate expression in the enteric nervous system and weaker levels in the olfactory sensory placode and otic capsule. GFP expression in glia co-localized with anti-GFAP antibodies, but did not co-localize with the neuronal antibodies HuC/D or calretinin in mature neurons.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping