ZFIN ID: ZDB-PUB-060616-45
GFAP transgenic zebrafish
Bernardos, R.L., and Raymond, P.A.
Date: 2006
Source: Gene expression patterns : GEP   6(8): 1007-1013 (Journal)
Registered Authors: Bernardos, Rebecca, Raymond, Pamela
Keywords: Glial fibrillary acidic protein, Müller glia, Radial glia, CNS, PNS, Zebrafish, Transgenic, GFP, Enteric nervous system, Retina, Hindbrain, Development
MeSH Terms:
  • Animals
  • Animals, Genetically Modified*/embryology
  • Animals, Genetically Modified*/metabolism
  • Central Nervous System/embryology
  • Central Nervous System/metabolism
  • Embryo, Nonmammalian
  • Gene Expression
  • Glial Fibrillary Acidic Protein/genetics*
  • Green Fluorescent Proteins/metabolism
  • Neuroglia/metabolism
  • Tissue Distribution
  • Transgenes
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics
PubMed: 16765104 Full text @ Gene Expr. Patterns
FIGURES
ABSTRACT
We have generated transgenic zebrafish that express green fluorescent protein (GFP) in glial cells driven by the zebrafish glial fibrillary acidic protein (GFAP) regulatory elements. Transgenic lines Tg(gfap:GFP) were generated from three founders; the results presented here are from the mi2001 line. GFP expression was first visible in the living embryo at the tail bud-stage, then in the developing brain by the 5-somite-stage ( approximately 12h post-fertilization, hpf) and then spreading posteriorly along the developing spinal cord by the 12-somite stage ( approximately 15hpf). At 24hpf GFP-expressing cells were in the retina and lens. By 72hpf GFP expression levels were strong and localized to the glia of the brain, neural retina, spinal cord, and ventral spinal nerves, with moderate expression in the enteric nervous system and weaker levels in the olfactory sensory placode and otic capsule. GFP expression in glia co-localized with anti-GFAP antibodies, but did not co-localize with the neuronal antibodies HuC/D or calretinin in mature neurons.
ADDITIONAL INFORMATION