Gene duplication and functional divergence of the zebrafish insulin-like growth factor 1 receptors

Schlueter, P.J., Royer, T., Farah, M.H., Laser, B., Chan, S.J., Steiner, D.F., and Duan, C.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology   20(8): 1230-1232 (Journal)
Registered Authors
Chan, Shu Jin, Duan, Cunming
MeSH Terms
  • Animals
  • Cell Survival
  • Chromosome Mapping
  • Ear, Inner/embryology
  • Exons
  • Eye/embryology
  • Gene Duplication*
  • Gene Targeting
  • Heart/embryology
  • Humans
  • Insulin-Like Growth Factor I
  • Introns
  • Motor Neurons/cytology
  • Muscle Contraction
  • Receptor, IGF Type 1/antagonists & inhibitors
  • Receptor, IGF Type 1/genetics
  • Receptor, IGF Type 1/physiology
  • Signal Transduction
  • Somatomedins/genetics*
  • Somatomedins/physiology*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/physiology
  • Zebrafish Proteins/antagonists & inhibitors
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/physiology*
16705083 Full text @ FASEB J.
Insulin-like growth factor (IGF) 1 receptor (IGF1R)-mediated signaling plays key roles in growth, development, and physiology. Recent studies have shown that there are two distinct igf1r genes in zebrafish, termed igf1ra and igf1rb. In this study, we tested the hypothesis that zebrafish igf1ra and igf1rb resulted from a gene duplication event at the igf1r locus and that this has led to their functional divergence. The genomic structures of zebrafish igf1ra and igf1rb were determined and their loci mapped. While zebrafish igf1ra has 21 exons and is located on linkage group (LG) 18, zebrafish igf1rb has 22 exons and mapped to LG 7. There is a strong syntenic relationship between the two zebrafish genes and the human IGF1R gene. Using a MO-based loss-of-function approach, we show that both Igf1ra and Igf1rb are required for zebrafish embryo viability and proper growth and development. Although Igf1ra and Igf1rb demonstrated a large degree of functional overlap with regard to cell differentiation in the developing eye, inner ear, heart, and muscle, they also exhibited functional distinction involving a greater requirement for Igf1rb in spontaneous muscle contractility. These findings suggest that the duplicated zebrafish igf1r genes play largely overlapping but not identical functional roles in early development and provide novel insight into the functional evolution of the IGF1R/insulin receptor gene family.--Schlueter, P. J., Royer, T., Mohamed, H. F., Laser, B., Chan, S. J., Steiner, D. F., Duan, C. Gene duplication and functional divergence of the zebrafish insulin-like growth factor 1 receptors.
Genes / Markers
Show all Figures
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes