PUBLICATION
In vitro effects of recombinant zebrafish IFN on spring viremia of carp virus and infectious hematopoietic necrosis virus
- Authors
- Wang, L., Wang, L., Zhang, H.X., Zhang, J.H., Chen, W.H., Ruan, X.F., and Xia, C.
- ID
- ZDB-PUB-060531-1
- Date
- 2006
- Source
- Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 26(4): 256-259 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Carps
- Cell Line
- Chick Embryo
- Chickens
- Escherichia coli/genetics
- Escherichia coli/metabolism
- Infectious hematopoietic necrosis virus/drug effects*
- Interferon Type I/biosynthesis
- Interferon Type I/genetics
- Interferon Type I/pharmacology*
- Molecular Sequence Data
- Recombinant Proteins
- Vesiculovirus/drug effects*
- Zebrafish Proteins/biosynthesis
- Zebrafish Proteins/genetics
- Zebrafish Proteins/pharmacology*
- PubMed
- 16704302 Full text @ J. Interferon Cytokine Res.
Citation
Wang, L., Wang, L., Zhang, H.X., Zhang, J.H., Chen, W.H., Ruan, X.F., and Xia, C. (2006) In vitro effects of recombinant zebrafish IFN on spring viremia of carp virus and infectious hematopoietic necrosis virus. Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research. 26(4):256-259.
Abstract
In order to evaluate the effects of fish recombinant interferon (rIFN) on fish pathogenic rhabdoviruses, the zebrafish (Danio rerio) IFN (DreIFN) allele B gene was cloned and expressed in Escherichia coli. In addition, the effects of recombinant DreIFN (rDreIFN) on spring viremia of carp virus (SVCV), infectious hematopoietic necrosis virus (IHNV), and vesicular stomatitis virus (VSV) were surveyed in fish and chicken cells. The mature peptide of DreIFN allele B gene encodes 163 amino acids. Residues 3 and 98 are a pair of cysteines that likely form an intrachain disulfide bridge. rDreIFN protein was detected as a band at 21.6 kDa by SDS-PAGE. The purified rDreIFN has anti-SVCV and anti-IHNV activity of 3 x 10(4) U/mg-10(7) U/mg. The results indicate that rDreIFN has higher activity against SVCV and IHNV on epithelioma papulosum cyprinid (EPC) than on grass carp (C. idellus) ovary (CO) cell lines and no activity against VSV on chick embryo fibroblasts (CEF).
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping