ZFIN ID: ZDB-PUB-060526-5
Mechanisms underlying long- and short-range nodal signaling in Zebrafish
Jing, X.H., Zhou, S.M., Wang, W.Q., and Chen, Y.
Date: 2006
Source: Mechanisms of Development   123(5): 388-394 (Journal)
Registered Authors: Chen, Yu
Keywords: Zebrafish, Nodal, Signaling range
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Body Patterning
  • Embryo, Nonmammalian
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Intracellular Signaling Peptides and Proteins
  • Left-Right Determination Factors
  • Molecular Sequence Data
  • Nodal Signaling Ligands
  • Protein Structure, Tertiary
  • Signal Transduction*
  • Transforming Growth Factor beta/genetics
  • Transforming Growth Factor beta/metabolism*
  • Zebrafish/embryology
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 16701984 Full text @ Mech. Dev.
Precise regulation of the signaling range of secreted molecules is essential for proper pattern formation during development. The Nodal family of TGF-beta proteins has been shown to function as both short- and long-range signals. But the underlying mechanisms remain elusive. In this study, we investigated the regulation of the signaling range of zebrafish Nodal proteins Cyclops and Squint, which are short- and long-range signals, respectively. We show that (1) the stability of Cyclops and Squint correlates with the activity range but increasing the stability of the short-range Cyclops does not increase its signaling range; (2) structural differences in the N-terminus region of the mature peptides of Cyclops and Squint determine their differences in the signaling range and swapping the N-terminus region of the Squint mature ligand into that of Cyclops makes the latter function at a distance.