ZFIN ID: ZDB-PUB-060403-6
Identification of calcium channel alpha1 subunit mRNA expressed in retinal bipolar neurons
Logiudice, L., Henry, D., and Matthews, G.
Date: 2006
Source: Molecular Vision 12: 184-189 (Journal)
Registered Authors:
Keywords: none
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Calcium Channels/genetics*
  • Cloning, Molecular
  • Goldfish
  • RNA, Messenger/metabolism*
  • Retina/cytology
  • Retina/metabolism*
  • Retinal Bipolar Cells/metabolism*
  • Reverse Transcriptase Polymerase Chain Reaction
PubMed: 16568031
PURPOSE: Glutamate release from goldfish bipolar cell terminals is driven by Ca2+ influx through L-type calcium channels that exhibit several uncommon features, including rapid kinetics of activation and deactivation, slow inactivation, and activation at an unusually negative voltage range for L-type channels. The purpose of this study was to establish the molecular identities of the alpha1 subunits responsible for these distinctive properties. METHODS: Transcripts for calcium channel alpha1 subunits expressed in individual goldfish ON-type bipolar cells were identified using single-cell reverse transcriptase polymerase chain reaction (RT-PCR). After cloning the goldfish homologs of the zebrafish and mammalian subunits, we designed sets of nested primers that are specific for Cav1.3a, and Cav1.3b L-type calcium channels. RESULTS: Large-terminal, ON-type bipolar cells express transcripts of Cav1.3a and/or Cav1.3b. CONCLUSIONS: The endogenous expression of only one or both subunits in a single cell raises the possibility of functionally distinct classes of bipolar cells that differ in calcium current properties.