PUBLICATION

Semaphorin3D guides retinal axons along the dorsoventral axis of the tectum

Authors
Liu, Y., Berndt, J., Su, F., Tawarayama, H., Shoji, W., Kuwada, J.Y., and Halloran, M.C.
ID
ZDB-PUB-060327-1
Date
2004
Source
The Journal of neuroscience : the official journal of the Society for Neuroscience   24(2): 310-318 (Journal)
Registered Authors
Berndt, Jason, Halloran, Mary, Kuwada, John, Liu, Yan, Shoji, Wataru, Su, Fengyun
Keywords
none
MeSH Terms
  • Animals
  • Antisense Elements (Genetics)
  • Axons/ultrastructure*
  • Carrier Proteins/genetics
  • Carrier Proteins/physiology*
  • Nerve Tissue Proteins/genetics
  • Nerve Tissue Proteins/physiology*
  • Neuropilins/metabolism
  • Organisms, Genetically Modified
  • Retina/embryology*
  • Retinal Ganglion Cells/cytology*
  • Semaphorins/genetics
  • Semaphorins/physiology*
  • Superior Colliculi/cytology*
  • Superior Colliculi/embryology
  • Superior Colliculi/metabolism
  • Visual Pathways/cytology
  • Visual Pathways/embryology
  • Zebrafish/anatomy & histology
  • Zebrafish/embryology
  • Zebrafish/genetics
PubMed
14724229 Full text @ J. Neurosci.
Abstract
We examined the role of Sema3D, a semaphorin of previously unknown function, in guiding retinal ganglion cell (RGC) axons to the optic tectum in the developing zebrafish. Sema3D is expressed more strongly in the ventral versus dorsal tectum, suggesting that it may participate in guiding RGC axons along the dorsoventral axis of the tectum. Ubiquitous misexpression of Sema3D in transgenic zebrafish inhibits ventral but not dorsal RGC axon growth. In addition, ventral RGC axons avoid or stop at individual cells misexpressing Sema3D along their pathway. Sema3D ubiquitous misexpression at later stages also causes ventral RGC axon arbors to spread more widely along the dorsoventral axis of the tectum. Knock-down of Sema3D with morpholino antisense causes ventral RGC axons to extend aberrantly into the ventral tectum. These results suggest that Sema3D in the ventral tectum normally acts to inhibit ventral RGCs from extending into ventral tectum, ensuring their correct innervation of dorsal tectum.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes