PUBLICATION

Identification of alternatively spliced dab1 isoforms in zebrafish

Authors
Costagli, A., Felice, B., Guffanti, A., Wilson, S.W., and Mione, M.
ID
ZDB-PUB-060315-7
Date
2006
Source
Development genes and evolution   216(6): 291-299 (Journal)
Registered Authors
Mione, Marina, Wilson, Steve
Keywords
Disabled1, Neuronal migration, Transcriptional variants, Reelin, Tyrosine phosphorylation
MeSH Terms
  • Adaptor Proteins, Signal Transducing
  • Alternative Splicing*
  • Animals
  • Animals, Genetically Modified
  • Base Sequence
  • Codon, Initiator
  • Codon, Terminator
  • DNA, Complementary
  • Exons
  • Gene Expression Regulation
  • Humans
  • In Situ Hybridization
  • Introns
  • Mice
  • Molecular Sequence Data
  • Nerve Tissue Proteins/genetics*
  • Nerve Tissue Proteins/metabolism*
  • Neurons/physiology
  • Phylogeny
  • Protein Isoforms/genetics
  • Protein Isoforms/metabolism
  • Species Specificity
  • Tyrosine/genetics
  • Zebrafish/genetics*
PubMed
16520940 Full text @ Dev. Genes Evol.
Abstract
We have investigated the genomic organization, the occurrence of alternative splicing and the differential expression of the zebrafish disabled1 (dab1) gene. Dab1 is a key effector of the Reelin pathway, which regulates neuronal migration during brain development in vertebrates. The coding region of the zebrafish dab1 gene spans over 600 kb of genomic DNA and is composed of 15 exons. Alternative splicing in a region enriched for tyrosine residues generates at least three different isoforms. These isoforms are developmentally regulated and show differential tissue expression. Comparison with mouse and human data shows an overall conservation of the genomic organization with different alternative splicing events generating species-specific isoforms. Because these alternative splicing events give rise to isoforms with different numbers of phosphorylateable tyrosines, we speculate that alternative splicing of the dab1 gene in zebrafish and in other vertebrates regulates the nature of the cellular response to the Reelin signal.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping