PUBLICATION

Cloning and embryonic expression of five distinct sfrp genes in the zebrafish Danio rerio

Authors
Tendeng, C., and Houart, C.
ID
ZDB-PUB-060313-6
Date
2006
Source
Gene expression patterns : GEP   6(8): 761-771 (Journal)
Registered Authors
Houart, Corinne, Tendeng, Christian
Keywords
Secreted frizzled-related protein, sFRP, Tlc, Sizzled, Wnt antagonist, Anterior neural plate, Telencephalon, Eyes, Hypothalamus, Midbrain, Tegmentum, Tectum, Hindbrain, Spinal cord, Otic vesicles, Lateral line, Somite, Adaxial cells, Gut, Fins
MeSH Terms
  • Animals
  • Central Nervous System/embryology
  • Central Nervous System/metabolism
  • Cloning, Molecular*/methods
  • Conserved Sequence
  • Embryo, Nonmammalian
  • Evolution, Molecular
  • Gastrula/metabolism
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental
  • Glycoproteins/genetics*
  • Glycoproteins/metabolism
  • Intercellular Signaling Peptides and Proteins/metabolism
  • Phylogeny
  • Proteins/genetics
  • Proteins/metabolism
  • Somites/metabolism
  • Spinal Cord/embryology
  • Spinal Cord/metabolism
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish Proteins/metabolism
PubMed
16504595 Full text @ Gene Expr. Patterns
Abstract
Recently, a new member of the secreted frizzled-related protein (sFRP) family, named tlc, has been identified as expressed by the anterior neural border (ANB) cells in the zebrafish Danio rerio. Tlc plays an important role in telencephalic induction and patterning. In absence of Tlc, formation of the telencephalon is severely delayed, but not abolished. This prompted us to clone the other zebrafish sfrp family members and analyse their expression patterns, in search of a family member that may partly functionally overlap with Tlc. Except sizzled, expression profile of sfrp genes in zebrafish has not been reported so far. Here, we describe the cloning of full-length cDNA for sfrp1a, sfrp1b, sfrp2, sfrp3 and sfrp5 gene transcripts and we examine their expression at different embryonic stages. Only sfrp1a is expressed in the anterior neural plate including the ANB cells where and when tlc is expressed. Interestingly, compared to both tlc and sfrp1a, wnt genes are complementary expressed more posteriorly in the neural plate. Later, both sfrp1a and sfrp5 expression profiles are overlapping, in particular at pharyngula stage these genes are expressed in the ventral part of the forebrain, midbrain and hindbrain. sfrp1b, sfrp2 and sfrp3 are mainly expressed in mesodermal and endodermal embryonic tissues. Expression profiles of these different genes in zebrafish gave interesting clues on the possible function and evolution of sFRPs in zebrafish and other organisms.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes