ZFIN ID: ZDB-PUB-060216-5
Two GC-rich boxes in huC promoter play distinct roles in controlling its neuronal specific expression in zebrafish embryos
Zhao, C., He, X., Tian, C., and Meng, A.
Date: 2006
Source: Biochemical and Biophysical Research Communications   342(1): 214-220 (Journal)
Registered Authors: Meng, Anming, Zhao, Chengtian
Keywords: Zebrafish, huC, Neuron, GC-rich box, Sp1-like transcription factors
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • ELAV Proteins/genetics*
  • ELAV Proteins/metabolism*
  • ELAV-Like Protein 3
  • Embryo, Nonmammalian/embryology
  • Embryo, Nonmammalian/metabolism
  • GC Rich Sequence/genetics*
  • Gene Expression Regulation, Developmental
  • Genes, Reporter/genetics
  • Molecular Sequence Data
  • Neurons/metabolism*
  • Organ Specificity
  • Promoter Regions, Genetic/genetics*
  • Sp1 Transcription Factor/metabolism
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism*
PubMed: 16472769 Full text @ Biochem. Biophys. Res. Commun.
HuC, a vertebrate ortholog of Drosophila elav gene, encodes an RNA binding protein and is involved in early neurogenesis. Zebrafish huC is expressed in distinct neurons, including Rohon-Beard (RB) sensory neurons, interneurons and motoneurons, during primary neurogenesis, and in all neurons later during secondary neurogenesis. In this study, we identify two GC-rich box elements, proximal GC (p-GC) box from -172 to -149 and distal GC (d-GC) box from -218 to -208, in zebrafish huC promoter. Using transgenic approach, we demonstrate that deletion of the p-GC box from the promoter results in loss of expression of the reporter GFP in neurons while deletion of the d-GC box leads to GFP expression only in dorsal RB sensory neurons. These results suggest that the p-GC box alone confers transcriptional activity of huC promoter in primary RB neurons and the d-GC is required for huC transcription in the full spectrum of spinal cord neurons. Further studies are needed to identify specific Sp1-like transcription factors that bind to these GC boxes and activate huC transcription.