header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-PUB-060130-10
Adenomatous polyposis coli-deficient zebrafish are susceptible to digestive tract neoplasia
Haramis, A.P., Hurlstone, A., van der Velden, Y., Begthel, H., van den Born, M., Offerhaus, G.J., and Clevers, H.C.
Date: 2006
Source: EMBO reports 7(4): 444-449 (Journal)
Registered Authors: Clevers, Hans, Haramis, Anna-Pavlina
Keywords: none
MeSH Terms:
  • Adenoma/genetics
  • Adenoma/metabolism*
  • Adenoma/pathology*
  • Adenomatous Polyposis Coli Protein/deficiency*
  • Adenomatous Polyposis Coli Protein/genetics
  • Adenomatous Polyposis Coli Protein/metabolism*
  • Animals
  • Animals, Genetically Modified
  • Digestive System Neoplasms/genetics
  • Digestive System Neoplasms/metabolism*
  • Digestive System Neoplasms/pathology*
  • Digestive System Neoplasms/veterinary
  • Gene Expression Regulation, Neoplastic
  • Zebrafish/genetics
  • Zebrafish/metabolism*
PubMed: 16439994 Full text @ EMBO Rep.
FIGURES
ABSTRACT
Truncation of the tumour suppressor adenomatous polyposis coli (APC) constitutively activates the Wnt/beta-catenin signalling pathway. This event constitutes the primary transforming event in sporadic colorectal cancer in humans. Moreover, humans or mice carrying germline truncating mutations in APC develop large numbers of intestinal adenomas. Here, we report that zebrafish that are heterozygous for a truncating APC mutation spontaneously develop intestinal, hepatic and pancreatic neoplasias that are highly proliferative, accumulate beta-catenin and express Wnt target genes. Treatment with the chemical carcinogen 7,12-dimethylbenz[a]anthracene accelerates the induction of these lesions. These observations establish apc-mutant zebrafish as a bona fide model for the study of digestive tract cancer.
ADDITIONAL INFORMATION