PUBLICATION
Expression of acetylcholinesterase (AChE) and aryl acylamidase (AAA) during early zebrafish embryogenesis
- Authors
- Allebrandt, K.V., Rajesh, V., and Layer, P.G.
- ID
- ZDB-PUB-060124-27
- Date
- 2005
- Source
- Chemico-biological interactions 157-158: 353-355 (Journal)
- Registered Authors
- Keywords
- Acetylcholinesterase, Aryl acylamidase, Serotonin
- MeSH Terms
-
- Acetylcholinesterase/genetics
- Acetylcholinesterase/metabolism*
- Amidohydrolases/genetics
- Amidohydrolases/metabolism*
- Animals
- Gene Expression Regulation, Developmental*/drug effects
- Gene Expression Regulation, Enzymologic*/drug effects
- Physostigmine/pharmacology
- Serotonin/pharmacology
- Time Factors
- Zebrafish/embryology*
- Zebrafish/metabolism*
- PubMed
- 16425445 Full text @ Chem. Biol. Interact.
Citation
Allebrandt, K.V., Rajesh, V., and Layer, P.G. (2005) Expression of acetylcholinesterase (AChE) and aryl acylamidase (AAA) during early zebrafish embryogenesis. Chemico-biological interactions. 157-158:353-355.
Abstract
In zebrafish, the onset of acetylcholinesterase (AChE) expression was detected by RT-PCR at 4 hpf (hours post-fertilization). The aryl acylamidase (AAA) associated with AChE, a serotonin sensitive activity with unknown physiological function, was significantly higher than the esterase activity on zebrafish embryos homogenates at 4–12 h development (test-t = 3.523; d.f. = 4). Remarkably, the ratio of AAA/AChE activity decreased 210-fold from 4 to 144 h development, indicating a distinct embryonic role of AAA during early embryogenesis. The AAA activity was sensitive to eserine and serotonin, ensuring its association with AChE. This is the first report of AAA activity on fish, establishing zebrafish as a model to study AAA on development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping