ZFIN ID: ZDB-PUB-060105-3
In situ detection of miRNAs in animal embryos using LNA-modified oligonucleotide probes
Kloosterman, W.P., Wienholds, E., de Bruijn, E., Kauppinen, S., and Plasterk, R.H.
Date: 2006
Source: Nature Methods 3(1): 27-29 (Journal)
Registered Authors: Kloosterman, Wigard, Plasterk, Ronald H.A., Wienholds, Erno, de Bruijn, Ewart
Keywords: none
MeSH Terms: Animals; Embryo, Mammalian/chemistry*; Embryo, Nonmammalian/chemistry; Gene Expression Profiling/methods; In Situ Hybridization/methods* (all 11) expand
PubMed: 16369549 Full text @ Nat. Methods
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ABSTRACT
MicroRNAs (miRNAs) are 20-23 nucleotide (nt) RNA molecules that regulate gene expression post-transcriptionally. A key step toward understanding the function of the hundreds of miRNAs identified in animals is to determine their expression during development. Here we performed a detailed analysis of conditions for in situ detection of miRNAs in the zebrafish embryo using locked nucleic acid (LNA)-modified DNA probes and report expression patterns for 15 miRNAs in the mouse embryo.
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