PUBLICATION
Delta and mu opioid receptors from the brain of a urodele amphibian, the rough-skinned newt Taricha granulosa: Cloning, heterologous expression, and pharmacological characterization
- Authors
- Bradford, C.S., Walthers, E.A., Stanley, D.J., Baugh, M.M., and Moore, F.L.
- ID
- ZDB-PUB-060105-11
- Date
- 2006
- Source
- General and comparative endocrinology 146(3): 276-290 (Journal)
- Registered Authors
- Keywords
- Delta, Mu, Opioid receptor, G protein, Brain, Amphibian, Salamander, Newt
- MeSH Terms
-
- Humans
- Animals
- Cloning, Molecular
- Phylogeny
- Receptors, Opioid, delta/genetics*
- Receptors, Opioid, delta/metabolism
- Enkephalin, Ala(2)-MePhe(4)-Gly(5)-/metabolism
- Amino Acid Sequence
- Base Sequence
- Enkephalin, D-Penicillamine (2,5)-/metabolism
- beta-Endorphin/metabolism
- Molecular Sequence Data
- COS Cells
- Enkephalin, Methionine/metabolism
- Brain Chemistry*
- Chlorocebus aethiops
- Sequence Alignment
- Receptors, Opioid, mu/genetics*
- Receptors, Opioid, mu/metabolism
- Naloxone/metabolism
- Salamandridae/physiology*
- Enkephalin, Leucine/metabolism
- PubMed
- 16375901 Full text @ Gen. Comp. Endocrinol.
Citation
Bradford, C.S., Walthers, E.A., Stanley, D.J., Baugh, M.M., and Moore, F.L. (2006) Delta and mu opioid receptors from the brain of a urodele amphibian, the rough-skinned newt Taricha granulosa: Cloning, heterologous expression, and pharmacological characterization. General and comparative endocrinology. 146(3):276-290.
Abstract
Two full-length cDNAs, encoding delta (delta) and mu (mu) opioid receptors, were cloned from the brain of the rough-skinned newt Taricha granulosa, complementing previous work from our laboratory describing the cloning of newt brain kappa (kappa) and ORL1 opioid receptors. The newt delta receptor shares 82% amino acid sequence identity with a frog delta receptor and lower (68-70%) identity with orthologous receptors cloned from mammals and zebrafish. The newt mu receptor shares 79% sequence identity with a frog mu receptor, 72% identity with mammalian mu receptors, and 66-69% identity with mu receptors cloned from teleost fishes. Membranes isolated from COS-7 cells transiently expressing the newt delta receptor possessed a single, high-affinity (K(d)=2.4nM) binding site for the nonselective opioid antagonist [(3)H]naloxone. In competition binding assays, the newt delta receptor displayed highest affinity for Met-enkephalin, relatively low affinity for Leu-enkephalin, beta-endorphin, and [d-penicillamine, d-penicillamine] enkephalin (DPDPE) (a delta-selective agonist in mammals), and very low affinity for mu-, kappa-, or ORL1-selective agonists. COS-7 cells expressing the newt mu receptor also possessed a high-affinity (K(d)=0.44nM) naloxone-binding site that showed highest affinity for beta-endorphin, moderate-to-low affinity for Met-enkephalin and Leu-enkephalin and DAMGO (a mu-selective agonist in mammals), and very low affinity for DPDPE and kappa- or ORL1-selective agonists. COS-7 cells expressing either receptor type (delta or mu) showed very high affinity (K(d)=0.1-0.3nM) for the nonselective opioid antagonist diprenorphine. Taricha granulosa expresses the same four subtypes (delta, mu, kappa, and ORL1) of opioid receptors found in other vertebrate classes, but ligand selectivity appears less stringent in the newt than has been documented in mammals.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping