PUBLICATION
Proton pump-rich cell is secreting acid in skin of zebrafish larvae (Danio rerio)
- Authors
- Lin, L.Y., Horng, J.L., Kunkel, J.G., and Hwang, P.P.
- ID
- ZDB-PUB-050913-3
- Date
- 2006
- Source
- American journal of physiology. Cell physiology 290(2): C371-C378 (Journal)
- Registered Authors
- Hwang, Pung Pung
- Keywords
- H+ ATPase, ionocytes, epithelial transport, ion-selective electrode
- MeSH Terms
-
- Acids*
- Animals
- Concanavalin A/metabolism
- Electrophysiology/methods
- Enzyme Inhibitors/metabolism
- In Situ Hybridization
- Larva*/anatomy & histology
- Larva*/metabolism
- Macrolides/metabolism
- Proton Pumps/metabolism*
- Proton-Translocating ATPases/genetics
- Proton-Translocating ATPases/metabolism
- Skin/cytology*
- Skin/metabolism
- Sodium-Potassium-Exchanging ATPase/genetics
- Sodium-Potassium-Exchanging ATPase/metabolism
- Zebrafish/anatomy & histology*
- Zebrafish/physiology
- Zebrafish Proteins/metabolism
- PubMed
- 16148031 Full text @ Am. J. Physiol. Cell Physiol.
Citation
Lin, L.Y., Horng, J.L., Kunkel, J.G., and Hwang, P.P. (2006) Proton pump-rich cell is secreting acid in skin of zebrafish larvae (Danio rerio). American journal of physiology. Cell physiology. 290(2):C371-C378.
Abstract
The mammalian kidney excretes its metabolic acid load through the proton transporting cells, intercalated cells, in the distal nephron and collecting duct. Fish excrete acid through external organs, gill or skin, however the cellular function is still controversial. In this work, molecular and electrophysiological approaches were used to identify a novel cell type secreting acid in skin of zebrafish larvae. Among keratinocytes covering larval surface, novel proton-secreting ionocytes, proton pump (H(+) ATPase)-rich cells, were identified to generate strong outward H(+) flux. The present work demonstrates for the first time, using non-invasive technique, H(+) secreting cells in an intact model animal, the zebrafish, showing it to be a suitable model to study the functions of vertebrate transporting epithelia in vivo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping