|ZFIN ID: ZDB-PUB-050711-3|
Inositol polyphosphates regulate zebrafish left-right asymmetry
Sarmah, B., Latimer, A.J., Appel, B., and Wente, S.R.
|Source:||Developmental Cell 9(1): 133-145 (Journal)|
|Registered Authors:||Appel, Bruce, Latimer, Andrew|
|PubMed:||15992547 Full text @ Dev. Cell|
Sarmah, B., Latimer, A.J., Appel, B., and Wente, S.R. (2005) Inositol polyphosphates regulate zebrafish left-right asymmetry. Developmental Cell. 9(1):133-145.
ABSTRACTVertebrate body plans have a conserved left-right (LR) asymmetry manifested in the position and anatomy of the heart, visceral organs, and brain. Recent studies have suggested that LR asymmetry is established by asymmetric Ca(2+) signaling resulting from cilia-driven flow of extracellular fluid across the node. We report here that inositol 1,3,4,5,6-pentakisphosphate 2-kinase (Ipk1), which generates inositol hexakisphosphate, is critical for normal LR axis determination in zebrafish. Zebrafish embryos express ipk1 symmetrically during gastrulation and early segmentation. ipk1 knockdown by antisense morpholino oligonucleotide injection randomized LR-specific gene expression and organ placement, effects that were associated with reduced intracellular Ca(2+) flux in cells surrounding the ciliated Kupffer's vesicle, a structure analogous to the mouse node. Our data suggest that the pathway for inositol hexakisphosphate production is a key regulator of asymmetric Ca(2+) flux during LR specification.