PUBLICATION

MicroRNA Expression in Zebrafish Embryonic Development

Authors
Wienholds, E., Kloosterman, W.P., Miska, E., Alvarez-Saavedra, E., Berezikov, E., de Bruijn, E., Horvitz, R.H., Kauppinen, S., and Plasterk, R.H.
ID
ZDB-PUB-050603-3
Date
2005
Source
Science (New York, N.Y.)   309(5732): 310-311 (Journal)
Registered Authors
Kloosterman, Wigard, Plasterk, Ronald H.A., Wienholds, Erno
Keywords
none
Datasets
GEO:GSE2628, GEO:GSE2625
MeSH Terms
  • Animals
  • Blotting, Northern
  • Embryo, Nonmammalian/metabolism*
  • Embryonic Development
  • Gene Expression*
  • In Situ Hybridization
  • MicroRNAs/genetics*
  • MicroRNAs/metabolism*
  • Multigene Family
  • Oligonucleotide Array Sequence Analysis
  • Oligonucleotide Probes
  • Organ Specificity
  • Time Factors
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish/metabolism
PubMed
15919954 Full text @ Science
Abstract
MicroRNAs (miRNAs) are small noncoding RNAs, approximately 21 nucleotides in length, that can regulate gene expression by base-pairing to partially complementary mRNAs. Regulation by miRNAs can play essential roles in embryonic development. We determined the temporal and spatial expression patterns of 115 conserved vertebrate miRNAs in zebrafish embryos by microarrays and by in situ hybridizations, using locked-nucleic-acid-modified oligonucleotide probes. Most miRNAs were expressed in a highly tissue-specific manner during segmentation and later stages, but not early in development, suggesting that their role is not in tissue fate establishment but in differentiation or maintenance of tissue identity.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping