ZFIN ID: ZDB-PUB-050518-3
bloodthirsty, an RBCC/TRIM gene required for erythropoiesis in zebrafish
Yergeau, D.A., Cornell, C.N., Parker, S.K., Zhou, Y., and Detrich, H.W. 3rd.
Date: 2005
Source: Developmental Biology 283(1): 97-112 (Journal)
Registered Authors: Detrich, H. William, Zhou, Yi
Keywords: Chaenocephalus aceratus; Notothenia coriiceps; Danio rerio; B30.2; bloodthirsty (bty) gene; Icefish; Rockcod; Zebrafish; Erythropoiesis; Hematopoiesis; RING-B box-coiled-coil (RBCC); Tripartite motif (TRIM)
MeSH Terms: Amino Acid Sequence; Animals; Base Sequence; Carrier Proteins/chemistry; Carrier Proteins/genetics* (all 22) expand
PubMed: 15890331 Full text @ Dev. Biol.
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ABSTRACT
The Antarctic icefishes (family Channichthyidae, suborder Notothenioidei) constitute the only vertebrate taxon that fails to produce red blood cells. These fishes can be paired with closely related, but erythrocyte-producing, notothenioids to discover erythropoietic genes via representational difference analysis. Using a B30.2-domain-encoding DNA probe so derived from the hematopoietic kidney (pronephros) of a red-blooded Antarctic rockcod, Notothenia coriiceps, we discovered a related, novel gene, bloodthirsty (bty), that encoded a 547-residue protein that contains sequential RING finger, B Box, coiled-coil, and B30.2 domains. bty mRNA was expressed by the pronephric kidney of N. coriiceps at a steady-state level 10-fold greater than that found in the kidney of the icefish Chaenocephalus aceratus. To test the function of bty, we cloned the orthologous zebrafish gene from a kidney cDNA library. Whole-mount in situ hybridization of zebrafish embryos showed that bty mRNA was present throughout development and, after the mid-blastula transition, was expressed in the head and in or near the site of primitive erythropoiesis in the tail just prior to red cell production. One- to four-cell embryos injected with two distinct antisense morpholino oligonucleotides (MOs) targeted to the 5'-end of the bty mRNA failed to develop red cells, whereas embryos injected with 4- and 5-bp mismatch control MOs produced wild-type quantities of erythrocytes. The morphant phenotype was rescued by co-injection of synthetic bty mRNA containing an artificial 5'-untranslated region (UTR) with the antisense MO that bound the 5'-UTR of the wild-type bty transcript. Furthermore, the expression of genes that mark terminal erythroid differentiation was greatly reduced in the antisense-MO-treated embryos. We conclude that bty is likely to play a role in differentiation of the committed red cell progenitor.
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