PUBLICATION
Identification and characterization of rat Dact1 and Dact2 genes in silico
- Authors
- Katoh, M., and Katoh, M.
- ID
- ZDB-PUB-050510-7
- Date
- 2005
- Source
- International journal of molecular medicine 15(6): 1045-1049 (Journal)
- Registered Authors
- Katoh, Masaru
- Keywords
- none
- MeSH Terms
-
- Adaptor Proteins, Signal Transducing
- Amino Acid Sequence
- Animals
- Base Sequence
- Carrier Proteins
- Chromosome Mapping
- Chromosomes
- Computational Biology
- Conserved Sequence
- Exons
- Expressed Sequence Tags
- Introns
- Molecular Sequence Data
- Neoplasm Proteins
- Nuclear Proteins/chemistry
- Protein Structure, Tertiary
- Proteins/chemistry*
- Proteins/genetics*
- Proteome/analysis*
- Proteomics
- Rats
- Sequence Homology, Amino Acid
- Sequence Homology, Nucleic Acid
- PubMed
- 15870912 Full text @ Int. J. Mol. Med.
Citation
Katoh, M., and Katoh, M. (2005) Identification and characterization of rat Dact1 and Dact2 genes in silico. International journal of molecular medicine. 15(6):1045-1049.
Abstract
DACT1 (DAPPER1), Frizzled receptors, MUSK receptor, VANGL1, VANGL2, PRICKLE1, PRICKLE2, DAAM1, Casein kinases, MARK3 (PAR1), PP2C, AXIN1, AXIN2, NKD1, NKD2, FRAT1, FRAT2 and CXXC4 are WNT signaling molecules associating with Dishevelled family proteins. Human DACT1 is the ortholog of Xenopus Dapper and Frodo, and human DACT2 (DAPPER2) is the paralog of human DACT1. Here, we identified and characterized rat Dact1 (Dapper1) and Dact2 (Dapper2) genes by using bioinformatics. Rat Dact1 gene, consisting of four exons, was located within AC136677.3 genome sequence. Rat Dact2 gene, consisting of four exons, was located within AC139434.3 genome sequence. Dact1 was mapped to rat chromosome 6q24, and Dact2 gene to rat chromosome 1q12. Rat Dact1 (778 aa) showed 93.7, 82.9, 60.3, 58.7 and 48.6% total-amino-acid identity with mouse Dact1, human DACT1, Xenopus Dapper, Xenopus Frodo and zebrafish dact1, respectively. Rat Dact2 (768 aa) showed 86.6, 59.6 and 38.3% total-amino-acid identity with mouse Dact2, human DACT2 and zebrafish dact2, respectively. Dact1 orthologs were more evolutionarily conserved than Dact2 orthologs. Seven DAPH domains (DAPH1-DAPH7), originally identified as the regions conserved between human DACT1 and DACT2, were conserved among mammalian Dact1 orthologs and Dact2 orthologs. DAPH2 domain, corresponding to the Leucine zipper motif, was located within the coiled-coil region. DAPH3 domain was the Serine rich region. DAPH7 domain was the C-terminal PDZ binding region. This is the first report on the rat Dact1 and Dact2 genes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping