ZFIN ID: ZDB-PUB-050427-7
roundabout4 is essential for angiogenesis in vivo
Bedell, V.M., Yeo, S.Y., Park, K.W., Chung, J., Seth, P., Shivalingappa, V., Zhao, J., Obara, T., Sukhatme, V.P., Drummond, I.A., Li, D.Y., and Ramchandran, R.
Date: 2005
Source: Proceedings of the National Academy of Sciences of the United States of America   102(18): 6373-6378 (Journal)
Registered Authors: Drummond, Iain, Obara, Tomoko, Ramchandran, Ramani, Sukhatme, Vikas, Yeo, Sang-Yeob
Keywords: axon guidance; endothelial cell; zebrafish
MeSH Terms:
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Computational Biology
  • DNA Primers
  • Endothelium/physiology
  • Gene Expression*
  • Genetic Complementation Test
  • Immunohistochemistry
  • In Situ Hybridization
  • Molecular Sequence Data
  • Neovascularization, Physiologic/genetics*
  • Neovascularization, Physiologic/physiology
  • Oligonucleotides
  • Receptors, Cell Surface/genetics
  • Receptors, Cell Surface/metabolism*
  • Sequence Homology
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 15849270 Full text @ Proc. Natl. Acad. Sci. USA
Stereotypical patterns of vascular and neuronal networks suggest that specific genetic programs tightly control path determination and, consequently, angiogenesis and axon-guidance mechanisms. Our study focuses on one member of the roundabout family of receptors, which traditionally mediate repulsion from the midline. Here, we characterize a fourth member of this family, roundabout4 (robo4), which is the predominant roundabout (robo) that is expressed in embryonic zebrafish vasculature. Gene knockdown and overexpression approaches show that robo4 is essential for coordinated symmetric and directed sprouting of intersomitic vessels and provide mechanistic insights into this process. Also, human robo4 gene functionally compensates for loss of robo4 gene function, suggesting evolutionary conservation. This article reports an endothelial-specific function for a robo gene in vertebrates in vivo.