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ZFIN ID: ZDB-PUB-050413-4
Integrinalpha5-Dependent Fibronectin Accumulation for Maintenance of Somite Boundaries in Zebrafish Embryos
Koshida, S., Kishimoto, Y., Ustumi, H., Shimizu, T., Furutani-Seiki, M., Kondoh, H., and Takada, S.
Date: 2005
Source: Developmental Cell 8(4): 587-598 (Journal)
Registered Authors: Furutani-Seiki, Makoto, Kishimoto, Yasuyuki, Kondoh, Hisato, Koshida, Sumito, Takada, Shinji
Keywords: none
MeSH Terms:
  • Animals
  • Body Patterning*
  • Cell Polarity
  • Ephrin-B2/genetics
  • Ephrin-B2/metabolism
  • Epithelium/embryology
  • Fibronectins/genetics
  • Fibronectins/metabolism*
  • Gene Expression Regulation, Developmental
  • In Situ Hybridization
  • Integrin alpha5/genetics
  • Integrin alpha5/metabolism*
  • Morphogenesis
  • Oligonucleotides, Antisense/genetics
  • Oligonucleotides, Antisense/metabolism
  • Somites/cytology*
  • Somites/physiology*
  • Zebrafish/anatomy & histology
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
PubMed: 15809040 Full text @ Dev. Cell
FIGURES
ABSTRACT
Boundary formation and epithelialization are crucial processes in the morphological segmentation of vertebrate somites. By a genetic screening procedure with zebrafish, we identified two genes, integrinalpha5 (itga5) and fibronectin (fn), required for these processes. Fibronectin proteins accumulate at somite boundaries in accordance with epithelialization of the somites. Both Fibronectin accumulation and the epithelialization are dependent on itga5, which is expressed in the most medial part of somites. Although somite boundaries are initially formed, but not maintained, in the anterior trunk of the mutant embryos deficient in either gene, their maintenance is defective at all axial levels of embryos deficient for both of these genes. Therefore, Integrinalpha5-directed assembly of Fibronectin appears critical for epithelialization and boundary maintenance of somites. Furthermore, with an additional deficiency in ephrin-B2a, the segmental defect in itga5 or fn mutant embryos is expanded posteriorly, indicating that both Integrin-Fibronectin and Eph-Ephrin systems function cooperatively in maintaining somite boundaries.
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