ZFIN ID: ZDB-PUB-050328-6
Isolation and Characterization of Ornithine Decarboxylase Gene from Flounder (Paralichthys olivaceus)
Lee, J.H., Son, M.Y., Yoon, M.Y., Choi, J.D., and Kim, Y.T.
Date: 2004
Source: Marine biotechnology (New York, N.Y.) 6(5): 453-462 (Journal)
Registered Authors:
Keywords: ornithine decarboxylase (ODC) - pyridoxal 5-phosphate (PLP) - flounder (Paralichthys olivaceus)
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain/enzymology
  • Chromatography, Affinity
  • Cluster Analysis
  • DNA Primers
  • DNA, Complementary/genetics
  • Embryo, Nonmammalian/enzymology
  • Flounder/genetics*
  • Gene Expression*
  • Kidney/enzymology
  • Liver/enzymology
  • Molecular Sequence Data
  • Ornithine Decarboxylase/genetics*
  • Phylogeny*
  • RNA, Messenger/metabolism*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology
PubMed: 15791490 Full text @ Mar. Biotechnol.
ABSTRACT
Ornithine decarboxylase (ODC) is a homodimeric enzyme dependent on pyridoxal 5'-phosphate. We identified a complementary DNA clone corresponding to ODC from the brain of adult flounder (Paralichthys olivaceus). The flounder ODC cDNA consisted of 2939 bp encoding 272 amino acid residues. The flounder ODC showed 80.3% sequence identity to zebrafish and 70.8% to rat at the amino acid level. Comparison of the structure and nucleotide sequence of the ODC genes revealed that the gene is highly conserved in the flounder, zebrafish, and rat. The presence of ODC mRNA species in brain, kidney, liver, and embryo was confirmed using the reverse transcriptase polymerase chain reaction. The recombinant protein of flounder ODC containing a short histidine tag at the carboxyl terminus was overexpressed in Escherichia coli BL21 (DE3) codon plus using an inducible T7 expression system, and was purified by Ni-NTA affinity chromatography.
ADDITIONAL INFORMATIONNo data available