PUBLICATION
Molecular cloning and expression of two closely related GTP-binding proteins from zebrafish
- Authors
- Wei, D., Yao, J., Yang, X., Cheng, L., Lu, D., and Xue, J.
- ID
- ZDB-PUB-050104-1
- Date
- 2004
- Source
- DNA sequence : the journal of DNA sequencing and mapping 15(4): 246-250 (Journal)
- Registered Authors
- Yang, Xueyan, Yao, Jihua
- Keywords
- DRG, GTP-binding protein, Zebrafish, Mid-blastula transition
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Cloning, Molecular
- DNA, Complementary
- GTP-Binding Proteins/genetics*
- GTP-Binding Proteins/metabolism
- Molecular Sequence Data
- Ovum/metabolism
- Phylogeny
- Zebrafish/genetics
- Zebrafish/metabolism
- PubMed
- 15620211 Full text @ DNA Seq.
Citation
Wei, D., Yao, J., Yang, X., Cheng, L., Lu, D., and Xue, J. (2004) Molecular cloning and expression of two closely related GTP-binding proteins from zebrafish. DNA sequence : the journal of DNA sequencing and mapping. 15(4):246-250.
Abstract
Developmentally regulated GTP-binding proteins (DRGs) are a subclass of GTP-binding proteins that have been discovered recently. Here we report two zebrafish DRG cDNA clones closely related to human and mouse DRG genes. The two DRG sequences showed a high degree of similarity (55% identity, 72% similarity) at the amino acids level. Whole mount in situ hybridization revealed expression of zebrafish DRGs maternally, following the onset of zygotic transcription at the mid-blastula transition (MBT) and throughout embryonic. The expression of these two genes in different tissues follows a similar pattern, suggesting that they may serve a similar function.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping