PUBLICATION
Developmental expression of aquaporin-3 in zebrafish embryos (Danio rerio)
- Authors
- Lance, S.L., Peterson, A.S., and Hagedorn, M.
- ID
- ZDB-PUB-041111-9
- Date
- 2004
- Source
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 138(3): 251-258 (Journal)
- Registered Authors
- Hagedorn, Mary
- Keywords
- Cryobiology; Cryopreservation; Teleost fish; Embryos; Zebrafish; Water permeability; Cryoprotectant permeability; Aquaporins
- MeSH Terms
-
- Animals
- Aquaporin 3
- Aquaporins/metabolism*
- Culture Media
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/embryology
- Embryo, Nonmammalian/metabolism
- Gene Expression Regulation, Developmental*/drug effects
- Microscopy, Confocal
- Osmolar Concentration
- Sodium Chloride/pharmacology
- Sucrose/pharmacology
- Survival Rate
- Zebrafish/embryology*
- Zebrafish/metabolism*
- PubMed
- 15533783 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
Citation
Lance, S.L., Peterson, A.S., and Hagedorn, M. (2004) Developmental expression of aquaporin-3 in zebrafish embryos (Danio rerio). Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 138(3):251-258.
Abstract
Fish embryos have never been successfully cryopreserved because of the low permeability of cryoprotectants into the yolk. Recently, we used aquaporin-3 fused with a green fluorescent protein (AQP3GFP) to modify the zebrafish embryo, and demonstrated that the pores functioned physiologically. This increased the water and cryoprotectant permeability of the membranes. We have continued our work on AQP3-modified embryos and here we report their developmental expression of AQP3, the success of various culture media on their survival and development, and their reproductive success. The AQP3GFP expression begins within 30 m after the mRNA AQP3GFP injection into the yolk of the 1- to 4-cell embryo. This expression is distributed in the membranes throughout the blastoderm and the yolk syncytial layer within 24 h. It diminishes after 96 h. We found no difference in the survival or normal development of embryos from AQP3GFP or wild-type adults. Additionally, zebrafish embryos did not require special culture medium to survive after AQP3GFP modification. In fact, they survived best in embryo medium (ca. 40 mOsm). Embryos reared entirely in embryo medium had a higher percent survival and a higher percent normal development than those exposed to a high osmolality sucrose culture medium (ca. 330 mOsm). The mechanism whereby these embryos can maintain their internal osmolality in a hypoosmotic solution with water channels in their membranes is unknown.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping