PUBLICATION
Induction of reversible hemolytic anemia in living zebrafish using a novel small molecule
- Authors
- Shafizadeh, E., Peterson, R.T., and Lin, S.
- ID
- ZDB-PUB-041111-8
- Date
- 2004
- Source
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 138(3): 245-249 (Journal)
- Registered Authors
- Lin, Shuo, Peterson, Randall, Shafizadeh, Ebrahim
- Keywords
- Zebrafish; Small molecules; Hemolytic anemia; Hematopoiesis; Phenylhydrazine; Heinz bodies; GATA1; Transgenic zebrafish
- MeSH Terms
-
- Anemia, Hemolytic/chemically induced*
- Anemia, Hemolytic/drug therapy
- Anemia, Hemolytic/pathology*
- Animals
- Disease Models, Animal
- Drug Evaluation, Preclinical/methods*
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/embryology
- Embryo, Nonmammalian/pathology
- Erythroid Cells/drug effects
- Erythroid Cells/pathology
- Erythropoiesis/drug effects
- Furans/chemistry
- Furans/pharmacology*
- Molecular Structure
- Phenylhydrazines
- Propionates/chemistry
- Propionates/pharmacology*
- Zebrafish/physiology*
- PubMed
- 15533782 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
Citation
Shafizadeh, E., Peterson, R.T., and Lin, S. (2004) Induction of reversible hemolytic anemia in living zebrafish using a novel small molecule. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 138(3):245-249.
Abstract
We used zebrafish to screen and identify small molecules that affect the process of vertebrate hematopoietic development. Zebrafish embryos were exposed to a library of 5000 synthetic compounds and screened for defects in primitive erythropoiesis. Here, we present the characterization of hemolytic anemia induced in zebrafish by the small molecule 5115318 (3-[5-methyl-furan 2-yl]-propionic acid N'-phenyl-hydrazide). This compound is capable of generating hemoglobin aggregates and Heinz bodies in red cells in vivo only. The induced anemia is reversible and treated fish recover in about 4 days. This study shows the feasibility of using zebrafish to screen for small molecules that can modulate the specific process of erythropoiesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping