Comparative cis-regulatory analyses identify new elements of the mouse Hoxc8 early enhancer
- Wang, W.C., Anand, S., Powell, D.R., Pawashe, A.B., Amemiya, C.T., and Shashikant, C.S.
- Journal of experimental zoology. Part B, Molecular and developmental evolution 302(5): 436-445 (Journal)
- Registered Authors
- Amemiya, Chris
- MeSH Terms
- Base Sequence
- Cloning, Molecular
- DNA Mutational Analysis
- DNA Primers
- Enhancer Elements, Genetic/genetics*
- Gene Expression Regulation, Developmental*
- Genes, Reporter/genetics
- Homeodomain Proteins/genetics*
- Molecular Sequence Data
- Regulatory Sequences, Nucleic Acid/genetics*
- Sequence Alignment
- 15384168 Full text @ J. Exp. Zool. B Mol. Dev. Evol.
Wang, W.C., Anand, S., Powell, D.R., Pawashe, A.B., Amemiya, C.T., and Shashikant, C.S. (2004) Comparative cis-regulatory analyses identify new elements of the mouse Hoxc8 early enhancer. Journal of experimental zoology. Part B, Molecular and developmental evolution. 302(5):436-445.
The Hoxc8 early enhancer is a 200 bp region that controls the early phase of Hoxc8 expression during mouse embryonic development. This enhancer defines the domain of Hoxc8 expression in the neural tube and mesoderm of the posterior regions of the developing embryo. Five distinct cis-acting elements, A-E, were previously shown to govern early phase Hoxc8 expression. Significant divergence between mammalian and fish Hoxc8 early enhancer sequences and activities suggested additional cis-acting elements. Here we describe four additional cis-acting elements (F-I) within the 200 bp Hoxc8 early enhancer region identified by comparative regulatory analysis and transgene-mutation studies. These elements affect posterior neural tube and mesoderm expression of the reporter gene, either singly or in combination. Surprisingly, these new elements are missing from the zebrafish and Fugu Hoxc8 early enhancer sequences. Considering that fish enhancers direct robust reporter expression in transgenic mouse embryos, it is tempting to postulate that fish and mammalian Hoxc8 early enhancers utilize different sets of elements to direct Hoxc8 early expression. These observations reveal a remarkable plasticity in the Hoxc8 early enhancer, suggesting different modes of initiation and establishment of Hoxc8 expression in different species. We postulate that extensive restructuring and remodeling of Hox cis-regulatory regions occurring in different taxa lead to relatively different Hox expression patterns, which in turn may act as a driving force in generating diverse axial morphologies. J. Exp. Zool. (Mol. Dev. Evol.) 302B:000-000, 2004. Copyright 2004 Wiley-Liss, Inc.
Genes / Markers
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes