ZFIN ID: ZDB-PUB-041004-20
STAT3 noncell-autonomously controls planar cell polarity during zebrafish convergence and extension
Miyagi, C., Yamashita, S., Ohba, Y., Yoshizaki, H., Matsuda, M., and Hirano, T.
Date: 2004
Source: The Journal of cell biology   166(7): 975-981 (Journal)
Registered Authors: Miyagi, Chiemi, Yamashita, Susumu
Keywords: STAT3; Dishevelled; Rho; planar cell polarity; zebrafish
MeSH Terms:
  • Adaptor Proteins, Signal Transducing
  • Animals
  • Cell Communication/genetics
  • Cell Movement/genetics*
  • Cell Polarity/genetics*
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism*
  • Down-Regulation/genetics
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/embryology
  • Embryo, Nonmammalian/metabolism
  • Gastrula/cytology
  • Gastrula/metabolism*
  • Gene Expression Regulation, Developmental/genetics
  • Mesoderm/cytology
  • Mesoderm/metabolism
  • Phosphoproteins/genetics
  • Phosphoproteins/metabolism
  • Proto-Oncogene Proteins/genetics
  • Proto-Oncogene Proteins/metabolism
  • STAT3 Transcription Factor
  • Signal Transduction/genetics
  • Trans-Activators/genetics
  • Trans-Activators/metabolism*
  • Wnt Proteins
  • Zebrafish/embryology*
  • Zebrafish/metabolism
  • Zebrafish Proteins
  • rhoA GTP-Binding Protein/genetics
  • rhoA GTP-Binding Protein/metabolism
PubMed: 15452141 Full text @ J. Cell Biol.
Zebrafish signal transducer and activator of transcription 3 (STAT3) controls the cell movements during gastrulation. Here, we show that noncell-autonomous activity of STAT3 signaling in gastrula organizer cells controls the polarity of neighboring cells through Dishevelled-RhoA signaling in the Wnt-planar cell polarity (Wnt-PCP) pathway. In STAT3-depleted embryos, although all the known molecules in the Wnt-PCP pathway were expressed normally, the RhoA activity in lateral mesendodermal cells was down-regulated, resulting in severe cell polarization defects in convergence and extension movements identical to Strabismus-depleted embryos. Cell-autonomous activation of Wnt-PCP signaling by DeltaN-dishevelled rescued the defect in cell elongation, but not the orientation of lateral mesendodermal cells in STAT3-depleted embryos. The defect in the orientation could be rescued by transplantation of shield cells having noncell-autonomous activity of STAT3 signaling. These results suggest that the cells undergoing convergence and extension movement may sense the gradient of signaling molecules, which are expressed in gastrula organizer by STAT3 and noncell-autonomously activate PCP signaling in neighboring cells during zebrafish gastrulation.