PUBLICATION
STAT3 noncell-autonomously controls planar cell polarity during zebrafish convergence and extension
- Authors
- Miyagi, C., Yamashita, S., Ohba, Y., Yoshizaki, H., Matsuda, M., and Hirano, T.
- ID
- ZDB-PUB-041004-20
- Date
- 2004
- Source
- The Journal of cell biology 166(7): 975-981 (Journal)
- Registered Authors
- Miyagi, Chiemi, Yamashita, Susumu
- Keywords
- STAT3; Dishevelled; Rho; planar cell polarity; zebrafish
- MeSH Terms
-
- Adaptor Proteins, Signal Transducing
- Animals
- Cell Communication/genetics
- Cell Movement/genetics*
- Cell Polarity/genetics*
- DNA-Binding Proteins/genetics
- DNA-Binding Proteins/metabolism*
- Down-Regulation/genetics
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/embryology
- Embryo, Nonmammalian/metabolism
- Gastrula/cytology
- Gastrula/metabolism*
- Gene Expression Regulation, Developmental/genetics
- Mesoderm/cytology
- Mesoderm/metabolism
- Phosphoproteins/genetics
- Phosphoproteins/metabolism
- Proto-Oncogene Proteins/genetics
- Proto-Oncogene Proteins/metabolism
- STAT3 Transcription Factor
- Signal Transduction/genetics
- Trans-Activators/genetics
- Trans-Activators/metabolism*
- Wnt Proteins
- Zebrafish/embryology*
- Zebrafish/metabolism
- Zebrafish Proteins
- rhoA GTP-Binding Protein/genetics
- rhoA GTP-Binding Protein/metabolism
- PubMed
- 15452141 Full text @ J. Cell Biol.
Citation
Miyagi, C., Yamashita, S., Ohba, Y., Yoshizaki, H., Matsuda, M., and Hirano, T. (2004) STAT3 noncell-autonomously controls planar cell polarity during zebrafish convergence and extension. The Journal of cell biology. 166(7):975-981.
Abstract
Zebrafish signal transducer and activator of transcription 3 (STAT3) controls the cell movements during gastrulation. Here, we show that noncell-autonomous activity of STAT3 signaling in gastrula organizer cells controls the polarity of neighboring cells through Dishevelled-RhoA signaling in the Wnt-planar cell polarity (Wnt-PCP) pathway. In STAT3-depleted embryos, although all the known molecules in the Wnt-PCP pathway were expressed normally, the RhoA activity in lateral mesendodermal cells was down-regulated, resulting in severe cell polarization defects in convergence and extension movements identical to Strabismus-depleted embryos. Cell-autonomous activation of Wnt-PCP signaling by DeltaN-dishevelled rescued the defect in cell elongation, but not the orientation of lateral mesendodermal cells in STAT3-depleted embryos. The defect in the orientation could be rescued by transplantation of shield cells having noncell-autonomous activity of STAT3 signaling. These results suggest that the cells undergoing convergence and extension movement may sense the gradient of signaling molecules, which are expressed in gastrula organizer by STAT3 and noncell-autonomously activate PCP signaling in neighboring cells during zebrafish gastrulation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping