PUBLICATION
Conservation of Bmp2 post-transcriptional regulatory mechanisms
- Authors
- Fritz, D.T., Liu, D., Xu, J., Jiang, S., and Rogers, M.B.
- ID
- ZDB-PUB-040914-7
- Date
- 2004
- Source
- The Journal of biological chemistry 279(47): 48950-48958 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- 3' Untranslated Regions
- Animals
- Base Sequence
- Bone Morphogenetic Protein 2
- Bone Morphogenetic Proteins/chemistry*
- Bone Morphogenetic Proteins/genetics*
- Calcium Phosphates/pharmacology
- Cell Differentiation
- Cell Line
- Cell Line, Tumor
- Chickens
- Cloning, Molecular
- Cricetinae
- Dogs
- Evolution, Molecular
- Expressed Sequence Tags
- Genes, Reporter
- Humans
- Luciferases/metabolism
- Mice
- Molecular Sequence Data
- Plasmids/metabolism
- Polyadenylation
- Polymerase Chain Reaction
- Promoter Regions, Genetic
- RNA Processing, Post-Transcriptional
- RNA, Messenger/metabolism
- Ribonucleases/metabolism
- Sequence Homology, Nucleic Acid
- Species Specificity
- Time Factors
- Transcription, Genetic
- Transfection
- Transforming Growth Factor beta/chemistry*
- Transforming Growth Factor beta/genetics*
- Tumor Necrosis Factor-alpha/metabolism
- Zebrafish
- PubMed
- 15358784 Full text @ J. Biol. Chem.
Citation
Fritz, D.T., Liu, D., Xu, J., Jiang, S., and Rogers, M.B. (2004) Conservation of Bmp2 post-transcriptional regulatory mechanisms. The Journal of biological chemistry. 279(47):48950-48958.
Abstract
Bone Morphogenetic Protein (BMP) orthologs from diverse species like flies and humans are functionally interchangeable and play key roles in fundamental processes such as dorso-ventral axis formation in metazoans. Because both transcriptional and post-transcriptional mechanisms play central roles in modulating developmental protein levels, we have analyzed the 3' untranslated region (3'UTR) of the Bmp 2 gene. This 3'UTR is unusually long and is alternatively polyadenylated. Mouse, human, and dog mRNAs are 83 to 87% identical within this region. A 265-nucleotide sequence, conserved between mammals, birds, frogs, and fish, is present in Bmp2, but not Bmp4. The ability of AmphiBMP2/4, a chordate ortholog to Bmp2 and Bmp4, to align with this sequence suggests that its function may have been lost in Bmp4. Activation of reporter genes by the conserved region acts by a post-transcriptional mechanism. Mouse, human, chick, and zebrafish Bmp2 synthetic RNAs decay rapidly in extracts from cells not expressing Bmp2. In contrast, these RNAs are relatively stable in extracts from Bmp2-expressing cells. Thus, Bmp2 RNA half-lives in vitro correlate with natural Bmp2 mRNA levels. The fact that non-murine RNAs interact appropriately with the mouse decay machinery suggests that the function of these cis-regulatory regions has been conserved for the 450 million years since the fish and tetrapod lineages diverged. Overall, our results suggest that the Bmp2 3'UTR contains essential regulatory elements that act post-transcriptionally.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping