ZFIN ID: ZDB-PUB-040819-2
Expression of zebrafish glutamate receptor delta2 in neurons with cerebellum-like wiring
Mikami, Y., Yoshida, T., Matsuda, N., and Mishina, M.
Date: 2004
Source: Biochemical and Biophysical Research Communications   322(1): 168-176 (Journal)
Registered Authors: Matsuda, Naoto, Mikami, Yoshinori, Mishina, Masayoshi, Yoshida, Tomoyuki
Keywords: Apical dendrite, Cerebellar Purkinje cell, Crest cell of the medial octavolateral nucleus, Glutamate receptor ?2, Neural wiring, Parallel fiber, Type I neuron of the optic tectum, Zebrafish
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Cerebellum/cytology*
  • Cerebellum/metabolism*
  • Computer Simulation
  • Dendrites/metabolism
  • Dendrites/ultrastructure
  • Models, Chemical
  • Molecular Sequence Data
  • Neural Pathways/cytology*
  • Neural Pathways/metabolism
  • Neurons/cytology*
  • Neurons/metabolism*
  • Protein Conformation
  • Purkinje Cells/cytology
  • Purkinje Cells/metabolism
  • Receptors, Glutamate/chemistry*
  • Receptors, Glutamate/genetics
  • Receptors, Glutamate/metabolism*
  • Receptors, Glutamate/ultrastructure
  • Recombinant Proteins/chemistry
  • Recombinant Proteins/metabolism
  • Recombinant Proteins/ultrastructure
  • Sequence Homology, Amino Acid
  • Tissue Distribution
  • Zebrafish
PubMed: 15313188 Full text @ Biochem. Biophys. Res. Commun.
Mammalian glutamate receptor (GluR) delta2 is selectively expressed in cerebellar Purkinje cells and plays key roles in cerebellar plasticity, motor learning, and neural wiring. Here, we isolated cDNA encoding the zebrafish ortholog of mammalian GluRdelta2. We found that in adult zebrafish brain, glurdelta2 mRNA was expressed not only in cerebellar Purkinje cells, but also in the crest cells of the medial octavolateral nucleus (MON) and the type I neurons of the optic tectum. Immunohistochemical analysis revealed that zebrafish GluRdelta2 proteins were selectively localized in the apical dendrites of these neurons. Interestingly, the crest cells of the MON and the type I neurons of the optic tectum receive large numbers of parallel fiber inputs at the apical dendrites and sensory inputs at the proximal or basal dendrites. These results suggest that the expression of zebrafish GluRdelta2 is selective for cerebellum-like neural wiring with large numbers of parallel fiber inputs.