PUBLICATION
Global changes in genomic methylation levels during early development of the zebrafish embryo
- Authors
- Mhanni, A.A., and McGowan, R.A.
- ID
- ZDB-PUB-040804-10
- Date
- 2004
- Source
- Development genes and evolution 214(8): 412-417 (Journal)
- Registered Authors
- McGowan, Ross
- Keywords
- Zebrafish, Methylation, Embryogenesis, Epigenetics
- MeSH Terms
-
- Animals
- Autoradiography
- Blotting, Southern
- DNA Methylation*
- DNA Primers
- DNA Probes
- Embryo, Nonmammalian/embryology
- Epigenesis, Genetic*
- Female
- Genome*
- Male
- Oocytes/metabolism
- Spermatozoa/metabolism
- Staining and Labeling
- Zebrafish/embryology*
- Zebrafish/genetics*
- PubMed
- 15309635 Full text @ Dev. Genes Evol.
Citation
Mhanni, A.A., and McGowan, R.A. (2004) Global changes in genomic methylation levels during early development of the zebrafish embryo. Development genes and evolution. 214(8):412-417.
Abstract
We have examined the methylation status of the zebrafish genome during early embryogenesis and we find evidence that methylation fluxes do occur in that organism. The parental genetic contributions to the zygote are, initially, differently methylated with the genome of the sperm being hypermethylated relative to the genome of the oocyte. Post-fertilization there is an immediate decrease in methylation of the embryonic genome but the methylation begins to increase rapidly and is re-established by the gastrulation stage. These results are consistent with the results of Santos et al. (Dev Biol 241:172-182, 2002), who examined the methylation of early mouse embryos, and this conservation argues that demethylation/re-methylation is an important part of vertebrate development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping