PUBLICATION

shocked Gene Is Required for the Function of a Premotor Network in the Zebrafish CNS

Authors
Cui, W.W., Saint-Amant, L., and Kuwada, J.Y.
ID
ZDB-PUB-040625-10
Date
2004
Source
Journal of neurophysiology   92(5): 2898-2908 (Journal)
Registered Authors
Cui, Wilson, Kuwada, John, Saint-Amant, Louis
Keywords
zebrafish, mutation, neural circuit, NMDA, behavior
MeSH Terms
  • Animals
  • Brain/physiology
  • Escape Reaction/physiology
  • Genes/physiology*
  • Motor Activity/physiology
  • Motor Cortex/physiology
  • Motor Neurons/physiology*
  • Muscle, Skeletal/innervation
  • Muscle, Skeletal/physiology
  • Nerve Net/physiology*
  • Zebrafish/embryology*
  • Zebrafish/genetics*
PubMed
15212431 Full text @ J. Neurophysiol.
Abstract
The analysis of behavioral mutations in zebrafish can be a powerful strategy for identifying genes that regulate the function and development of neural circuits in the vertebrate CNS. A neurophysiological analysis of the shocked (sho) mutation that affects the initiation of swimming following mechanosensory stimulation was undertaken to identify the function of the sho gene product in the developing motor circuitry. The cutaneous Rohon-Beard (RB) mechanosensory neurons responded normally to stimulation and muscle fibers were unaffected in sho embryos suggesting that the output of the CNS is abnormal. Indeed whole cell patch recordings from mutant muscle cells showed normal spontaneous miniature endplate potentials, but abnormal touch-evoked endplate potentials. Furthermore, motor neuron recordings showed that bursts of rhythmic action potentials from synaptically-dependent depolarizations are initiated in wildtype motor neurons following sensory stimulation or bath application of NMDA. These bursts presumably correspond to bouts of swimming. In sho motor neurons the touch-evoked depolarizations were not sustained resulting in an abbreviated burst of action potentials. The defective responses were not due to any obvious defect in sho motor neurons since their basic properties were normal. These results suggest that in sho embryos there is aberrant motor processing within the CNS and that normal motor processing requires the sho gene product.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes