PUBLICATION
shocked Gene Is Required for the Function of a Premotor Network in the Zebrafish CNS
- Authors
- Cui, W.W., Saint-Amant, L., and Kuwada, J.Y.
- ID
- ZDB-PUB-040625-10
- Date
- 2004
- Source
- Journal of neurophysiology 92(5): 2898-2908 (Journal)
- Registered Authors
- Cui, Wilson, Kuwada, John, Saint-Amant, Louis
- Keywords
- zebrafish, mutation, neural circuit, NMDA, behavior
- MeSH Terms
-
- Animals
- Brain/physiology
- Escape Reaction/physiology
- Genes/physiology*
- Motor Activity/physiology
- Motor Cortex/physiology
- Motor Neurons/physiology*
- Muscle, Skeletal/innervation
- Muscle, Skeletal/physiology
- Nerve Net/physiology*
- Zebrafish/embryology*
- Zebrafish/genetics*
- PubMed
- 15212431 Full text @ J. Neurophysiol.
Citation
Cui, W.W., Saint-Amant, L., and Kuwada, J.Y. (2004) shocked Gene Is Required for the Function of a Premotor Network in the Zebrafish CNS. Journal of neurophysiology. 92(5):2898-2908.
Abstract
The analysis of behavioral mutations in zebrafish can be a powerful strategy for identifying genes that regulate the function and development of neural circuits in the vertebrate CNS. A neurophysiological analysis of the shocked (sho) mutation that affects the initiation of swimming following mechanosensory stimulation was undertaken to identify the function of the sho gene product in the developing motor circuitry. The cutaneous Rohon-Beard (RB) mechanosensory neurons responded normally to stimulation and muscle fibers were unaffected in sho embryos suggesting that the output of the CNS is abnormal. Indeed whole cell patch recordings from mutant muscle cells showed normal spontaneous miniature endplate potentials, but abnormal touch-evoked endplate potentials. Furthermore, motor neuron recordings showed that bursts of rhythmic action potentials from synaptically-dependent depolarizations are initiated in wildtype motor neurons following sensory stimulation or bath application of NMDA. These bursts presumably correspond to bouts of swimming. In sho motor neurons the touch-evoked depolarizations were not sustained resulting in an abbreviated burst of action potentials. The defective responses were not due to any obvious defect in sho motor neurons since their basic properties were normal. These results suggest that in sho embryos there is aberrant motor processing within the CNS and that normal motor processing requires the sho gene product.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping