PUBLICATION
fgf17b, a novel member of Fgf family, helps patterning zebrafish embryos
- Authors
- Cao, Y., Zhao, J., Sun, Z., Zhao, Z., Postlethwait, J., and Meng, A.
- ID
- ZDB-PUB-040617-5
- Date
- 2004
- Source
- Developmental Biology 271(1): 130-143 (Journal)
- Registered Authors
- Cao, Ying, Meng, Anming, Postlethwait, John H., Sun, Zhihui, Zhao, Jue
- Keywords
- Fibroblast growth factor, Mesoderm, Dorsalization, Neuroectoderm, Zebrafish
- MeSH Terms
-
- Animals
- Base Sequence
- Blotting, Northern
- Body Patterning/genetics
- Body Patterning/physiology*
- Chromosome Mapping
- DNA Primers
- Fetal Proteins
- Fibroblast Growth Factor 3
- Fibroblast Growth Factor 8
- Fibroblast Growth Factors/genetics*
- Fibroblast Growth Factors/metabolism*
- Gene Expression Regulation, Developmental*
- Gene Library
- Glycoproteins/genetics
- Glycoproteins/metabolism
- In Situ Hybridization
- Intercellular Signaling Peptides and Proteins/genetics
- Intercellular Signaling Peptides and Proteins/metabolism
- Left-Right Determination Factors
- Microinjections
- Molecular Sequence Data
- Phylogeny
- Reverse Transcriptase Polymerase Chain Reaction
- T-Box Domain Proteins/metabolism
- Transforming Growth Factor beta/metabolism
- Zebrafish/embryology*
- Zebrafish Proteins/metabolism
- PubMed
- 15196956 Full text @ Dev. Biol.
Citation
Cao, Y., Zhao, J., Sun, Z., Zhao, Z., Postlethwait, J., and Meng, A. (2004) fgf17b, a novel member of Fgf family, helps patterning zebrafish embryos. Developmental Biology. 271(1):130-143.
Abstract
Fibroblast growth factors (Fgfs) play important roles in the pattern formation of early vertebrate embryos. We have identified a zebrafish ortholog of human FGF17, named fgf17b. The first phase of fgf17b expression occurs in the blastodermal margin of late blastulae and in the embryonic shield of early gastrulae. The second phase starts after the onset of segmentation, mainly in the presomitic mesoderm and newly formed somites. Injection of fgf17b mRNA into one-cell embryos induces expression of the mesodermal marker no tail (ntl) and rescues ntl expression suppressed by overexpression of lefty1 (lft1). Overexpression of fgf17b dorsalizes zebrafish gastrulae by enhancing expression of chordin (chd), which is an antagonist of the ventralizing signals BMPs. In addition, overexpression of fgf17b posteriorizes the neuroectoderm. Simultaneous knockdown of fgf17b and fgf8 with antisense morpholinos results in reduction of chd and ntl. Knockdown of fgf17b can alleviate inhibitory effect of ectopic expression of fgf3 on otx1. These data together suggest that Fgf17b plays a role in early embryonic patterning. We also demonstrate that fgf17b and fgf8 have stronger mesoderm inducting activity than fgf3, whereas fgf17b and fgf3 have stronger activity in posteriorizing the neuroectoderm than fgf8. Like fgf8, activation of fgf17b expression depends on Nodal signaling.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping