PUBLICATION
Fish as Bioreactors: Transgene Expression of Human Coagulation Factor VII in Fish Embryos
- Authors
- Hwang, G., Müller, F., Rahman, M.A., Williams, D.W., Murdock, P.J., Pasi, K.J., Goldspink, G., Farahmand, H., and Maclean, N.
- ID
- ZDB-PUB-040510-1
- Date
- 2004
- Source
- Marine biotechnology (New York, N.Y.) 6(5): 485-492 (Journal)
- Registered Authors
- Müller, Ferenc, Williams, Darren W.
- Keywords
- human coagulation factor VII, transgenic fish, bioreactor
- MeSH Terms
-
- Photometry
- Microinjections
- Blood Coagulation/drug effects
- Enzyme-Linked Immunosorbent Assay
- Zygote/metabolism*
- Fishes/metabolism*
- Promoter Regions, Genetic/genetics
- Bioreactors*
- Animals
- Genetic Vectors/genetics
- Cytomegalovirus/genetics
- DNA, Complementary/genetics
- Humans
- Transgenes/genetics*
- Factor VII/genetics
- Factor VII/metabolism*
- Factor VII/pharmacology
- PubMed
- 15129328 Full text @ Mar. Biotechnol.
Citation
Hwang, G., Müller, F., Rahman, M.A., Williams, D.W., Murdock, P.J., Pasi, K.J., Goldspink, G., Farahmand, H., and Maclean, N. (2004) Fish as Bioreactors: Transgene Expression of Human Coagulation Factor VII in Fish Embryos. Marine biotechnology (New York, N.Y.). 6(5):485-492.
Abstract
A plasmid containing human coagulation factor VII (hFVII) complementary DNA regulated by a cytomegalovirus promoter was microinjected into fertilized eggs of zebrafish, African catfish, and tilapia. The active form of hFVll was detected in the fish embryos by various assays. This positive expression of human therapeutic protein in fish embryos demonstrates the possibility of exploitation of transgenic fish as bioreactors.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping