ZFIN ID: ZDB-PUB-040429-5
On the relevance of genotoxicity for fish populations I: effects of a model genotoxicant on zebrafish (Danio rerio) in a complete life-cycle test
Diekmann, M., Hultsch, V., and Nagel, R.
Date: 2004
Source: Aquatic toxicology (Amsterdam, Netherlands)   68(1): 13-26 (Journal)
Registered Authors: Nagel, Roland
Keywords: Zebrafish, Life-cycle test, Population, 4-Nitroquinoline-1-oxide
MeSH Terms:
  • 4-Nitroquinoline-1-oxide/toxicity*
  • Analysis of Variance
  • Animals
  • Body Weights and Measures
  • Computer Simulation
  • Dose-Response Relationship, Drug
  • Gas Chromatography-Mass Spectrometry
  • Growth/drug effects*
  • Mutagenicity Tests
  • Mutagens/toxicity*
  • Reproduction/drug effects*
  • Toxicity Tests, Chronic
  • Zebrafish/genetics
  • Zebrafish/growth & development*
PubMed: 15110466 Full text @ Aquat. Toxicol.
Genotoxicity may be detected in surface waters by means of various genotoxicity assays. In order to enable an ecotoxicological assessment of the consequences of such genotoxic potential for fish populations, a complete life-cycle test with zebrafish (Danio rerio) and the model genotoxicant 4-nitroquinoline-1-oxide (NQO) was conducted. Zebrafish (f(1)) were continuously exposed to NQO (i.e. 0.1, 0.3, 1.1, 2.9, and 14.6microg/l, respectively) from fertilised eggs until sexual maturity. In addition to reproduction studies in the f(1)-generation, f(2)-fish were exposed to NQO during the first 6 weeks of development. Up to 2.9microg/l NQO, fish did not display differences in survival and growth [Formula: see text]. A NQO concentration of 14.6microg/l, however, was lethal. Female fish exposed to all NQO concentrations up to 2.9microg/l displayed a significant reduction in egg production [Formula: see text]. A mathematical simulation revealed that exposure to weak concentrations of NQO is leading to an elevated extinction risk.