PUBLICATION
On the relevance of genotoxicity for fish populations I: effects of a model genotoxicant on zebrafish (Danio rerio) in a complete life-cycle test
- Authors
- Diekmann, M., Hultsch, V., and Nagel, R.
- ID
- ZDB-PUB-040429-5
- Date
- 2004
- Source
- Aquatic toxicology (Amsterdam, Netherlands) 68(1): 13-26 (Journal)
- Registered Authors
- Nagel, Roland
- Keywords
- Zebrafish, Life-cycle test, Population, 4-Nitroquinoline-1-oxide
- MeSH Terms
-
- 4-Nitroquinoline-1-oxide/toxicity*
- Analysis of Variance
- Animals
- Body Weights and Measures
- Computer Simulation
- Dose-Response Relationship, Drug
- Gas Chromatography-Mass Spectrometry
- Growth/drug effects*
- Mutagenicity Tests
- Mutagens/toxicity*
- Reproduction/drug effects*
- Toxicity Tests, Chronic
- Zebrafish/genetics
- Zebrafish/growth & development*
- PubMed
- 15110466 Full text @ Aquat. Toxicol.
Citation
Diekmann, M., Hultsch, V., and Nagel, R. (2004) On the relevance of genotoxicity for fish populations I: effects of a model genotoxicant on zebrafish (Danio rerio) in a complete life-cycle test. Aquatic toxicology (Amsterdam, Netherlands). 68(1):13-26.
Abstract
Genotoxicity may be detected in surface waters by means of various genotoxicity assays. In order to enable an ecotoxicological assessment of the consequences of such genotoxic potential for fish populations, a complete life-cycle test with zebrafish (Danio rerio) and the model genotoxicant 4-nitroquinoline-1-oxide (NQO) was conducted. Zebrafish (f(1)) were continuously exposed to NQO (i.e. 0.1, 0.3, 1.1, 2.9, and 14.6microg/l, respectively) from fertilised eggs until sexual maturity. In addition to reproduction studies in the f(1)-generation, f(2)-fish were exposed to NQO during the first 6 weeks of development. Up to 2.9microg/l NQO, fish did not display differences in survival and growth [Formula: see text]. A NQO concentration of 14.6microg/l, however, was lethal. Female fish exposed to all NQO concentrations up to 2.9microg/l displayed a significant reduction in egg production [Formula: see text]. A mathematical simulation revealed that exposure to weak concentrations of NQO is leading to an elevated extinction risk.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping