PUBLICATION

Chemokine signaling regulates sensory cell migration in zebrafish

Authors
Li, Q., Shirabe, K., and Kuwada, J,Y.
ID
ZDB-PUB-040415-5
Date
2004
Source
Developmental Biology   269(1): 123-136 (Journal)
Registered Authors
Kuwada, John, Li, Qin
Keywords
Zebrafish, Chemokine, SDF-1, CXCR4, Migration, Lateral line, Transgenic, Morpholino knockdown, Odysseus
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Cell Movement/physiology*
  • Chemokine CXCL12
  • Chemokines, CXC/genetics*
  • Chemokines, CXC/metabolism
  • Oligonucleotides, Antisense
  • Receptors, CXCR4/genetics
  • Receptors, CXCR4/metabolism
  • Sensory Receptor Cells/embryology*
  • Sensory Receptor Cells/metabolism
  • Signal Transduction/physiology*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/physiology
PubMed
15081362 Full text @ Dev. Biol.
Abstract
Chemokines play an important role in the migration of a variety of cells during development. Recent investigations have begun to elucidate the importance of chemokine signaling within the developing nervous system. To better appreciate the neural function of chemokines in vivo, the role of signaling by SDF-1 through its CXCR4 receptor was analyzed in zebrafish. The SDF-1-CXCR4 expression pattern suggested that SDF-1-CXCR4 signaling was important for guiding migration by sensory cells known as the migrating primordium of the posterior lateral line. Ubiquitous induction of the ligand in transgenic embryos, antisense knockdown of the ligand or receptor, and a genetic receptor mutation all disrupted migration by the primordium. Furthermore, in embryos in which endogenous SDF-1 was knocked down, the primordium migrated towards exogenous sources of SDF-1. These data demonstrate that SDF-1 signaling mediated via CXCR4 functions as a chemoattractant for the migrating primordium and that chemokine signaling is both necessary and sufficient for directing primordium migration.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes