ZFIN ID: ZDB-PUB-040405-5
Visual pigment phosphorylation but not transducin translocation can contribute to light adaptation in zebrafish cones
Kennedy, M.J., Dunn, F.A., and Hurley, J.B.
Date: 2004
Source: Neuron   41(6): 915-928 (Journal)
Registered Authors: Hurley, James B.
Keywords: none
MeSH Terms:
  • Adaptation, Ocular/drug effects
  • Adaptation, Ocular/physiology*
  • Animals
  • Calcium/metabolism
  • Calcium/pharmacology
  • Calcium Signaling/drug effects
  • Calcium Signaling/physiology
  • Cytoplasm/drug effects
  • Cytoplasm/metabolism
  • Immunohistochemistry
  • Light
  • Mutation/genetics
  • Organ Culture Techniques
  • Phosphorylation/drug effects
  • Phosphorylation/radiation effects
  • Photic Stimulation
  • Protein Transport/physiology
  • Protein Transport/radiation effects
  • Retinal Cone Photoreceptor Cells/cytology
  • Retinal Cone Photoreceptor Cells/drug effects
  • Retinal Cone Photoreceptor Cells/metabolism*
  • Retinal Pigments/genetics
  • Retinal Pigments/metabolism*
  • Rod Opsins/genetics
  • Rod Opsins/metabolism
  • Transducin/metabolism*
  • Vision, Ocular/physiology*
  • Vision, Ocular/radiation effects
  • Zebrafish
PubMed: 15046724 Full text @ Neuron
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ABSTRACT
The ability of cone photoreceptors to adapt to light is extraordinary. In this study we evaluated two biochemical processes, visual pigment phosphorylation and transducin translocation, for their ability to contribute to light adaptation in zebrafish cones. Since cytoplasmic Ca(2+) regulates light adaptation, the sensitivities of these processes to both light and Ca(2+) were examined. Cytoplasmic Ca(2+) regulates the sites of light-stimulated phosphorylation. Unexpectedly, we found that Ca(2+) also regulates the extent of phosphorylation of unbleached cone pigments. Immunocytochemical analyses revealed that neither light nor cytoplasmic Ca(2+) influences the localization of transducin in zebrafish cones.
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