PUBLICATION

Developmental expression of the POU domain transcription factor Brn-3b (Pou4f2) in the lateral line and visual system of zebrafish

Authors
DeCarvalho, A.C., Cappendijk, S.L., and Fadool, J.M.
ID
ZDB-PUB-040326-1
Date
2004
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   229(4): 869-876 (Journal)
Registered Authors
Fadool, James M.
Keywords
none
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Brain/embryology*
  • Brain/metabolism
  • Cloning, Molecular
  • DNA-Binding Proteins/genetics*
  • DNA-Binding Proteins/isolation & purification
  • DNA-Binding Proteins/metabolism*
  • Embryo, Nonmammalian/metabolism
  • Eye/embryology*
  • Eye/metabolism
  • Gene Expression Regulation, Developmental
  • Molecular Sequence Data
  • RNA, Messenger/analysis
  • Retina/embryology
  • Retina/metabolism
  • Sequence Alignment
  • Superior Colliculi/embryology
  • Superior Colliculi/metabolism
  • Transcription Factor Brn-3
  • Transcription Factor Brn-3B
  • Transcription Factor Brn-3C
  • Transcription Factors/genetics*
  • Transcription Factors/isolation & purification
  • Transcription Factors/metabolism*
  • Zebrafish/embryology*
PubMed
15042710 Full text @ Dev. Dyn.
Abstract
Members of the class IV POU domain transcription factors are important regulators of neural development. In mouse, Brn-3b (Pou4f2, Brn3.2) and Brn-3c (Pou4f3, Brn3.1) are essential for the normal differentiation and maturation of retinal ganglion cells (RGCs) and hair cells of the auditory system, respectively. In this report, the cloning and expression profile of brn-3b in the zebrafish (Danio rerio) were assessed as the first step for understanding its role in the development of sensory systems. Two brn-3b alternative transcripts exhibited different onset of expression during development but shared overlapping expression domains in the adult visual system. The brn-3b expression in the zebrafish retina was consistent with a conserved role in differentiation and maintenance of RGCs. Expression was also observed in the optic tectum. Unexpectedly, brn-3b was prominently expressed in the migrating posterior lateral line primordium and larval neuromasts. For comparison, brn-3c expression was limited to the otic vesicle and was not detected in the lateral line during embryonic development. The expression of brn-3b in the mechanosensory lateral line of fish suggests a conserved function of a class IV POU domain transcription factor in sensory system development.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping