PUBLICATION

EphrinB2a in the zebrafish retinotectal system

Authors
Wagle, M., Grunewald, B., Subburaju, S., Barzaghi, C., Le Guyader, S., Chan, J., and Jesuthasan, S.
ID
ZDB-PUB-040311-2
Date
2004
Source
Journal of neurobiology   59(1): 57-65 (Journal)
Registered Authors
Chan, Joanne, Grunewald, Barbara, Jesuthasan, Suresh, Le Guyader, Sylvie, Subburaju, Sivan, Wagle, Mahendra
Keywords
ephrinB2. tectal neuron, stripe assay, mutant, neurogenic
MeSH Terms
  • Animals
  • Axons/metabolism
  • Axons/virology
  • Baculoviridae/physiology
  • Carbocyanines/metabolism
  • Embryo, Nonmammalian
  • Ephrin-A2/genetics
  • Ephrin-A2/metabolism*
  • Eye/metabolism
  • Eye/transplantation
  • Gene Expression Regulation, Developmental*
  • In Situ Hybridization/methods
  • In Vitro Techniques
  • Mutation
  • Organ Transplantation/methods
  • Retina/cytology
  • Retina/embryology
  • Retina/metabolism*
  • Retinal Ganglion Cells/metabolism
  • Superior Colliculi/cytology
  • Superior Colliculi/embryology
  • Superior Colliculi/metabolism*
  • Visual Pathways/embryology
  • Visual Pathways/metabolism*
  • Zebrafish
PubMed
15007827 Full text @ J. Neurobiol.
Abstract
Members of the Eph-B family of receptors tyrosine kinase and their transmembrane ligands have been implicated in dorsoventral patterning of the vertebrate retinotectal projection. In the zebrafish retinotectal system, however, ephrinB2a is expressed strongly in the posterior tectum, in tectal neurons that form physical contacts with retinal ganglion cell (RGC) axons. In the gnarled mutant, where tectal neurons form ectopically in the pretectum, RGC axons stall before entering the tectum, or else are misrouted or branch aberrantly in the tectal neuropil. Ectopic expression of ephrinB2a in the anterior midbrain of wild-type embryos, with the aid of baculovirus, also inhibits RGC axon entry into the tectum. In vitro, zebrafish RGC axons are repelled by stripes of purified ephrinB2a. It is proposed that ephrinB2a may signal a subpopulation of RGC axons that they have reached their target neurons in the tectum.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Errata and Notes