PUBLICATION
The POU domain protein spg (pou2/Oct4) is essential for endoderm formation in cooperation with the HMG domain protein casanova
- Authors
- Reim, G., Mizoguchi, T., Stainier, D.Y., Kikuchi, Y., and Brand, M.
- ID
- ZDB-PUB-040211-2
- Date
- 2004
- Source
- Developmental Cell 6(1): 91-101 (Journal)
- Registered Authors
- Brand, Michael, Kikuchi, Yutaka, Reim, Gerlinde, Stainier, Didier
- Keywords
- none
- MeSH Terms
-
- Zebrafish/embryology*
- Zebrafish/metabolism
- Male
- Body Patterning/genetics
- SOX Transcription Factors
- Transcription Factors/deficiency*
- Transcription Factors/genetics
- Transcription Factors/metabolism*
- DNA-Binding Proteins/genetics
- Animals
- Stem Cells/cytology
- Stem Cells/metabolism
- High Mobility Group Proteins/genetics
- High Mobility Group Proteins/metabolism*
- RNA, Messenger/pharmacology
- Gene Expression Regulation, Developmental/genetics
- Octamer Transcription Factor-3
- Zygote/cytology
- Zygote/metabolism
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/embryology*
- Embryo, Nonmammalian/metabolism
- Cell Lineage/genetics
- Endoderm/cytology
- Endoderm/metabolism*
- Promoter Regions, Genetic/genetics
- Female
- SOXF Transcription Factors
- Cell Differentiation/genetics
- Zebrafish Proteins*
- PubMed
- 14723850 Full text @ Dev. Cell
Citation
Reim, G., Mizoguchi, T., Stainier, D.Y., Kikuchi, Y., and Brand, M. (2004) The POU domain protein spg (pou2/Oct4) is essential for endoderm formation in cooperation with the HMG domain protein casanova. Developmental Cell. 6(1):91-101.
Abstract
The gastrulating vertebrate embryo develops three germlayers: ectoderm, mesoderm, and endoderm. Zebrafish endoderm differentiation starts with the activation of sox17 by casanova (cas). We report that spg (pou2/Oct4) is essential for endoderm formation. Embryos devoid of maternal and zygotic spg function (MZspg) lack endodermal precursors. Cell transplantations show that spg acts in early endodermal precursors, and cas mRNA-injection into MZspg embryos does not restore endoderm development. spg and cas together are both necessary and sufficient to activate endoderm development, and stimulate expression of a sox17 promoter-luciferase reporter. Endoderm and mesoderm derive from a common origin, mesendoderm. We propose that Spg and Cas commit mesendodermal precursors to an endodermal fate. The joint control of endoderm formation by spg and cas suggests that the endodermal germlayer may be a tissue unit with distinct genetic control, thus adding genetic support to the germlayer concept in metazoan development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping