PUBLICATION

Toll-like receptor gene family and TIR-domain adapters in Danio rerio

Authors
Jault, C., Pichon, L., and Chluba, J.
ID
ZDB-PUB-040109-34
Date
2004
Source
Molecular immunology   40(11): 759-771 (Journal)
Registered Authors
Keywords
Zebrafish; Innate immunity; Toll-like receptors; Evolution; Expression
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Evolution, Molecular
  • Membrane Glycoproteins/genetics*
  • Membrane Glycoproteins/physiology
  • Molecular Sequence Data
  • Multigene Family*
  • Phylogeny
  • Protein Structure, Tertiary
  • Receptors, Cell Surface/genetics*
  • Receptors, Cell Surface/physiology
  • Sequence Alignment
  • Sequence Analysis, Protein
  • Sequence Homology
  • Signal Transduction/genetics
  • Signal Transduction/physiology
  • Toll-Like Receptor 1
  • Toll-Like Receptor 2
  • Toll-Like Receptor 4
  • Toll-Like Receptor 8
  • Toll-Like Receptors
  • Zebrafish/genetics*
  • Zebrafish/physiology
PubMed
14687933 Full text @ Mol. Immunol.
Abstract
The toll-like family of receptors (TLR) is an ancient pattern recognition receptor family, conserved from insects to mammals. We have identified in zebrafish (Danio rerio) 19 putative TLR variants, the orthologs of mammalian TLR2-5, 7-9, a fish specific receptor type group and three putative splice variants. One receptor is very close to mammalian TLR1, 6 and 10 and seems to be their common ancestor. However, in contrast to the pufferfish, Fugu rubripes, we found two receptors homologous to TLR4, showing that lack of TLR4 is not general for fish. In addition, we identified two members close to mammalian TLR8 and five members close to FuguTLR21 and goldfish TLR, a TLR group which now has only been found in fish. By RT-PCR we showed that all TLR are widely expressed in adult tissues, but also at different stages of development. All these TLRs contain very conserved toll/interleukin-1 receptor (TIR) domains able to interact with TIR-domain of adapter molecules. We demonstrate here that TIR-domain containing adapters MyD88 and SARM are present in zebrafish, showing that TLR adapter molecules are highly conserved in evolution.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping