PUBLICATION
Zebrafish retinal slice preparation
- Authors
- Connaughton, V.P.
- ID
- ZDB-PUB-031229-8
- Date
- 2003
- Source
- Methods in cell science : an official journal of the Society for In Vitro Biology 25(1-2): 49-58 (Review)
- Registered Authors
- Connaughton, Victoria P.
- Keywords
- Bipolar cells, Physiology, Retinal slice, Technique, Zebrafish
- MeSH Terms
-
- Animals
- Electroretinography
- Eye/anatomy & histology*
- Eye/cytology
- Ion Channel Gating/physiology*
- Retina/anatomy & histology*
- Retina/physiology
- Zebrafish/anatomy & histology*
- Zebrafish/physiology
- PubMed
- 14739587 Full text @ Methods Cell Sci.
Citation
Connaughton, V.P. (2003) Zebrafish retinal slice preparation. Methods in cell science : an official journal of the Society for In Vitro Biology. 25(1-2):49-58.
Abstract
This paper describes the protocol for generating thin (~100 µm) slices of the zebrafish retina. Retinal slices retain the cytoarchitecture and synaptic contacts found in vivo, allowing neurons to be identified prior to physiological recordings. These characteristics distinguish retinal slices from both isolated cell and eyecup preparations. Studies using the zebrafish retinal slice have classified different retinal cell types, documented voltage- and ligand-gated current responses in distal bipolar neurons, and correlated physiological responses with neuronal morphology. Data collected using this protocol have provided baseline information about retinal circuitry that can be directly applied to behavioral studies examining visual function and/or mutants with visual system defects.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping