An SP1-like transcription factor Spr2 acts downstream of Fgf signaling to mediate mesoderm induction
- Zhao, J., Cao, Y., Zhao, C., Postlethwait, J., and Meng, A.
- The EMBO journal 22(22): 6078-6088 (Journal)
- Registered Authors
- Cao, Ying, Meng, Anming, Postlethwait, John H., Zhao, Chengtian, Zhao, Jue
- fibroblast growth factor, mesoderm, SP1-like transcription factor, zebrafish
- MeSH Terms
- DNA-Binding Proteins/genetics
- DNA-Binding Proteins/metabolism*
- Embryonic Induction/physiology*
- Fetal Proteins
- Fibroblast Growth Factors/metabolism*
- Gene Expression Regulation, Developmental/physiology
- Nodal Protein
- Proto-Oncogene Proteins/metabolism
- Sp Transcription Factors/genetics
- Sp Transcription Factors/metabolism*
- Sp3 Transcription Factor
- T-Box Domain Proteins/metabolism
- Transforming Growth Factor beta/genetics
- Transforming Growth Factor beta/metabolism
- Wnt Proteins
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- 14609954 Full text @ EMBO J.
Zhao, J., Cao, Y., Zhao, C., Postlethwait, J., and Meng, A. (2003) An SP1-like transcription factor Spr2 acts downstream of Fgf signaling to mediate mesoderm induction. The EMBO journal. 22(22):6078-6088.
Fgf signaling, mediated in part by the transcription factor Brachyury/Xbra/Ntl, plays important roles in mesoderm formation during the early development of vertebrate embryos. We have identified a zebrafish gene, spr2, which encodes a member of the Sp1-like transcription factor family. spr2 is expressed in both hypoblast and epiblast cells during late blastulation/early gastrulation, and in some mesodermal and neural tissues at later stages. Injection with spr2 mRNA enhances ntl expression and alleviates the inhibitory effect on ntl of XFD, a Xenopus dominant-negative FGF receptor. In contrast, morpholino- mediated knockdown of Spr2 activity inhibits ntl expression and reduces the inductive effect of Fgfs on ntl. We also demonstrate that Fgf signaling relays mesoderm induction activity of Nodal signaling and Spr2 is involved in this signal relay process. Furthermore, the correct spatial expression of spr2 requires Nodal, Fgf and Wnt signals. We suggest that expression of spr2 is an immediate-early response to mesoderm induction by Fgfs, which in turn regulates the expression of effector genes involved in the development of mesodermal tissues.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes