PUBLICATION
Mutagenesis study on the zebra fish SOX9 high-mobility group: comparison of sequence and non-sequence specific HMG domains
- Authors
- Hsiao, N.-W., Samuel, D., Liu, Y.-N., Chen, L.-C., Yang, T.-Y., Jayaraman, G., and Lyu, P.-C.
- ID
- ZDB-PUB-031105-2
- Date
- 2003
- Source
- Biochemistry 42(38): 11183-11193 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Circular Dichroism
- Escherichia coli/metabolism
- High Mobility Group Proteins/chemistry
- High Mobility Group Proteins/genetics*
- High Mobility Group Proteins/metabolism*
- Models, Molecular
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Oligonucleotide Probes/genetics
- Oligonucleotide Probes/metabolism
- Protein Denaturation
- Protein Structure, Secondary
- Protein Structure, Tertiary
- SOX9 Transcription Factor
- Sequence Alignment
- Sequence Homology, Amino Acid
- Transcription Factors/chemistry
- Transcription Factors/genetics*
- Transcription Factors/metabolism*
- Zebrafish
- PubMed
- 14503868 Full text @ Biochemistry
Citation
Hsiao, N.-W., Samuel, D., Liu, Y.-N., Chen, L.-C., Yang, T.-Y., Jayaraman, G., and Lyu, P.-C. (2003) Mutagenesis study on the zebra fish SOX9 high-mobility group: comparison of sequence and non-sequence specific HMG domains. Biochemistry. 42(38):11183-11193.
Abstract
A unique class of proteins, containing high-mobility group (HMG) domain(s), recognizes unusual DNA structures and/or bends specific to AT-rich linear double-stranded DNA. The DNA binding feature of these proteins is exhibited in the HMG domain(s). Although the sequence specific and non-sequence specific HMG domains exhibit very high degrees of sequence similarity, the reasons for the difference between their DNA recognition mechanisms are unclear. A series of zebra fish SOX9 HMG domain mutants was prepared in an effort to elucidate the importance of various residues on protein stability and DNA binding. This study is the first of a comprehensive mutagenesis study on a sequence specific HMG domain. Comparing how various residues influence sequence specific and non-sequence specific HMG domains helps us to rationalize their mode of action. Positively charged amino acids concentrated at the surface of sequence specific HMG domains recognize specific, linear AT-rich DNA segments. After the negative charges at the surface of the DNA are neutralized, the hydrophobic residues of the protein may intercalate DNA. Phenylalanine at position 12 plays a crucial role in the sequence specific HMG domain. The differences in pI values, the instability index, and DNA contact regions between sequence and non-sequence specific HMG domains are associated with their functional modes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping