ZFIN ID: ZDB-PUB-031103-3
Syndecan-2 is essential for angiogenic sprouting during zebrafish development
Chen, E., Hermanson, S., and Ekker, S.C.
Date: 2004
Source: Blood 103(5): 1710-1719 (Journal)
Registered Authors: Ekker, Stephen C., Hermanson, Spencer
Keywords: none
MeSH Terms: 5' Untranslated Regions; Amino Acid Sequence; Animals; Base Sequence; Chromosome Mapping (all 29) expand
PubMed: 14592839 Full text @ Blood
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ABSTRACT
We used a morpholino-based gene targeting screen to identify a novel protein essential for vascular development using the zebrafish, Danio rerio. We show that syndecan-2, a cell-surface heparan sulfate proteoglycan, is essential for angiogenic sprouting during embryogenesis. The vascular function of syndecan-2 is likely conserved, as zebrafish and mouse syndecan-2 show similar expression patterns around major trunk vessels, and human syndecan-2 can restore angiogenic sprouting in syndecan-2 morphants. In contrast, forced expression of a truncated form of syndecan-2 results in embryos with defects in angiogenesis, indicating the highly-conserved cytoplasmic tail is important for the vascular function of syndecan-2. We further show that VEGF and syndecan-2 genetically interact in vivo using both gain of function and loss of function studies in zebrafish. VEGF-mediated ectopic signaling is compromised in syndecan-2 morphants, and ectopic syndecan-2 potentiates ectopic VEGF signaling. Syndecan-2 as a novel angiogenic factor is a potential candidate for use in the development of angiogenesis-based therapies.
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