ZFIN ID: ZDB-PUB-031103-3
Syndecan-2 is essential for angiogenic sprouting during zebrafish development
Chen, E., Hermanson, S., and Ekker, S.C.
Date: 2004
Source: Blood 103(5): 1710-1719 (Journal)
Registered Authors: Ekker, Stephen C., Hermanson, Spencer
Keywords: none
MeSH Terms:
  • 5' Untranslated Regions
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chromosome Mapping
  • Cloning, Molecular
  • Cytoplasm/metabolism
  • Gene Expression Regulation, Developmental*
  • Green Fluorescent Proteins
  • Heparan Sulfate Proteoglycans/chemistry
  • Humans
  • In Situ Hybridization
  • Luminescent Proteins/metabolism
  • Membrane Glycoproteins/biosynthesis*
  • Membrane Glycoproteins/physiology*
  • Mice
  • Molecular Sequence Data
  • Neovascularization, Physiologic*
  • Phenotype
  • Phylogeny
  • Protein Binding
  • Proteoglycans/biosynthesis*
  • Proteoglycans/physiology*
  • RNA, Messenger/metabolism
  • Signal Transduction
  • Syndecan-2
  • Vascular Endothelial Growth Factor A/metabolism
  • Zebrafish
  • Zebrafish Proteins
PubMed: 14592839 Full text @ Blood
FIGURES
ABSTRACT
We used a morpholino-based gene targeting screen to identify a novel protein essential for vascular development using the zebrafish, Danio rerio. We show that syndecan-2, a cell-surface heparan sulfate proteoglycan, is essential for angiogenic sprouting during embryogenesis. The vascular function of syndecan-2 is likely conserved, as zebrafish and mouse syndecan-2 show similar expression patterns around major trunk vessels, and human syndecan-2 can restore angiogenic sprouting in syndecan-2 morphants. In contrast, forced expression of a truncated form of syndecan-2 results in embryos with defects in angiogenesis, indicating the highly-conserved cytoplasmic tail is important for the vascular function of syndecan-2. We further show that VEGF and syndecan-2 genetically interact in vivo using both gain of function and loss of function studies in zebrafish. VEGF-mediated ectopic signaling is compromised in syndecan-2 morphants, and ectopic syndecan-2 potentiates ectopic VEGF signaling. Syndecan-2 as a novel angiogenic factor is a potential candidate for use in the development of angiogenesis-based therapies.
ADDITIONAL INFORMATION