PUBLICATION
Insertional mutagenesis in zebrafish
- Authors
- Amsterdam, A.
- ID
- ZDB-PUB-031031-13
- Date
- 2003
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 228(3): 523-534 (Journal)
- Registered Authors
- Amsterdam, Adam
- Keywords
- zebrafish, retrovirus, vertebrate genetics, functional genomics, gene-trap
- MeSH Terms
-
- Animals
- DNA Transposable Elements*
- Gene Expression
- Models, Animal
- Mutagenesis, Insertional/genetics*
- Mutagenesis, Insertional/methods
- Retroviridae/genetics
- Zebrafish/genetics*
- Zebrafish Proteins/genetics*
- PubMed
- 14579390 Full text @ Dev. Dyn.
Citation
Amsterdam, A. (2003) Insertional mutagenesis in zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 228(3):523-534.
Abstract
Insertional mutagenesis is a method for identifying genes essential for a given biological process by using the integration of DNA as the mutagen, thereby facilitating the cloning of the mutated gene. The use of retrovirus-mediated insertional mutagenesis in zebrafish has led to the mutation and rapid identification of hundreds of genes required for embryonic development and cell viability and growth, revealing the diversity of gene products required for the development of this vertebrate. Here, I will review the methodology of this approach and the results to date, as well as other potential ways to use insertional mutagenesis for genetic screens.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping