ZFIN ID: ZDB-PUB-031009-4
The microRNA-producing enzyme Dicer1 is essential for zebrafish development
Wienholds, E., Koudijs, M.J., van Eeden, F.J., Cuppen, E., and Plasterk, R.H.
Date: 2003
Source: Nature Genetics 35(3): 217-218 (Journal)
Registered Authors: Cuppen, Edwin, Plasterk, Ronald H.A., van Eeden, Freek, Wienholds, Erno
Keywords: none
MeSH Terms:
  • Animals
  • DEAD-box RNA Helicases
  • Endoribonucleases/genetics
  • Endoribonucleases/physiology*
  • Gene Silencing
  • MicroRNAs/biosynthesis*
  • Mutation
  • RNA Helicases/genetics
  • RNA Helicases/physiology*
  • Ribonuclease III
  • Zebrafish/embryology*
PubMed: 14528306 Full text @ Nat. Genet.
ABSTRACT
MicroRNAs (miRNAs) are produced by the Dicer1 enzyme; the role of Dicer1 in vertebrate development is unknown. Here we report target-selected inactivation of the dicer1 gene in zebrafish. We observed an initial build-up of miRNA levels, produced by maternal Dicer1, in homozygous dicer1 mutants, but miRNA accumulation stopped after a few days. This resulted in developmental arrest around day 10. These results indicate that miRNA-producing Dicer1 is essential for vertebrate development.
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