PUBLICATION
The microRNA-producing enzyme Dicer1 is essential for zebrafish development
- Authors
- Wienholds, E., Koudijs, M.J., van Eeden, F.J., Cuppen, E., and Plasterk, R.H.
- ID
- ZDB-PUB-031009-4
- Date
- 2003
- Source
- Nature Genetics 35(3): 217-218 (Journal)
- Registered Authors
- Cuppen, Edwin, Plasterk, Ronald H.A., van Eeden, Freek, Wienholds, Erno
- Keywords
- none
- MeSH Terms
-
- Zebrafish/embryology*
- RNA Helicases/genetics
- RNA Helicases/physiology*
- Endoribonucleases/genetics
- Endoribonucleases/physiology*
- DEAD-box RNA Helicases
- Mutation
- MicroRNAs/biosynthesis*
- Gene Silencing
- Animals
- Ribonuclease III
- PubMed
- 14528306 Full text @ Nat. Genet.
Citation
Wienholds, E., Koudijs, M.J., van Eeden, F.J., Cuppen, E., and Plasterk, R.H. (2003) The microRNA-producing enzyme Dicer1 is essential for zebrafish development. Nature Genetics. 35(3):217-218.
Abstract
MicroRNAs (miRNAs) are produced by the Dicer1 enzyme; the role of Dicer1 in vertebrate development is unknown. Here we report target-selected inactivation of the dicer1 gene in zebrafish. We observed an initial build-up of miRNA levels, produced by maternal Dicer1, in homozygous dicer1 mutants, but miRNA accumulation stopped after a few days. This resulted in developmental arrest around day 10. These results indicate that miRNA-producing Dicer1 is essential for vertebrate development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping