PUBLICATION
Positional cloning of the young mutation identifies an essential role for the Brahma chromatin remodeling complex in mediating retinal cell differentiation
- Authors
- Gregg, R.G., Willer, G.B., Fadool, J.M., Dowling, J.E., and Link, B.A.
- ID
- ZDB-PUB-030527-14
- Date
- 2003
- Source
- Proceedings of the National Academy of Sciences of the United States of America 100(11): 6535-6540 (Journal)
- Registered Authors
- Dowling, John E., Fadool, James M., Gregg, Ronald G., Link, Brian, Willer, Greg
- Keywords
- none
- MeSH Terms
-
- Drosophila Proteins
- Chromatin/metabolism*
- Animals
- Cloning, Molecular
- Cell Cycle Proteins/genetics*
- Molecular Sequence Data
- Mutation*
- Trans-Activators/genetics*
- Base Sequence
- Zebrafish/embryology
- DNA Primers
- PubMed
- 12748389 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Gregg, R.G., Willer, G.B., Fadool, J.M., Dowling, J.E., and Link, B.A. (2003) Positional cloning of the young mutation identifies an essential role for the Brahma chromatin remodeling complex in mediating retinal cell differentiation. Proceedings of the National Academy of Sciences of the United States of America. 100(11):6535-6540.
Abstract
Zebrafish with the young (yng) mutation show a defect in retinal cell differentiation. Here we demonstrate that a mutation in a brahma-related gene (brg1) is responsible for the yng phenotype. Brahma homologues function as essential subunits for SWI/SNF-type chromatin remodeling complexes. Our analysis indicates that brg1 is required for the wave of mitogen-activated protein kinase activity that precedes retinal cell differentiation. Using specific inhibitors of the mitogen-activated protein kinase pathway we show this signal has a direct role in retinal cell differentiation. Lastly, through investigations of mutants in other chromatin remodeling subunits, we provide genetic evidence for gene and tissue specificity of the Brahma chromatin remodeling complex.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping