PUBLICATION

In vivo studies of liver-type fatty acid binding protein (L-FABP) gene expression in liver of transgenic zebrafish (Danio rerio)

Authors
Her, G.-M., Chiang, C.-C., Chen, W.-Y., and Wu, J.-L.
ID
ZDB-PUB-030319-9
Date
2003
Source
FEBS letters   538(1-3): 125-133 (Journal)
Registered Authors
Her, Guor Muor, Wu, Jen-Leih
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Base Sequence
  • Carrier Proteins/genetics*
  • Cloning, Molecular
  • DNA Primers
  • Fatty Acid-Binding Proteins
  • Green Fluorescent Proteins
  • Liver/metabolism*
  • Luminescent Proteins/metabolism
  • Neoplasm Proteins*
  • Nerve Tissue Proteins*
  • Polymerase Chain Reaction
  • Zebrafish
  • Zebrafish Proteins*
PubMed
12633865 Full text @ FEBS Lett.
Abstract
Mammalian liver fatty acid binding protein (L-FABP) is a small cytosolic protein in various tissues including liver, small intestine and kidney and is thought to play a crucial role in intracellular fatty acid trafficking and metabolism. To better understand its tissue-specific regulation during zebrafish hepatogenesis, we isolated 5'-flanking sequences of the zebrafish L-FABP gene and used a green fluorescent protein (GFP) transgenic strategy to generate liver-specific transgenic zebrafish. The 2.8-kb 5'-flanking sequence of zebrafish L-FABP gene was sufficient to direct GFP expression in liver primordia, first observed in 2 dpf embryos and then continuously to the adult stage. This pattern of transgenic expression is consistent with the expression pattern of the endogenous gene. F2 inheritance rates of 42-51% in all the seven transgenic lines were consistent with the ratio of Mendelian segregation. Further, hhex and zXbp-1 morphants displayed a visible liver defect, which suggests that it is possible to establish an in vivo system for screening genes required for liver development.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping